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The Chloroplast Genome and Its Application

A technology of chloroplast genome and Xia Ying, applied in the field of genes

Active Publication Date: 2021-03-09
ZHEJIANG FORESTRY ACAD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the research on the chloroplast genome of Xiaying cherry leaves is still blank

Method used

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  • The Chloroplast Genome and Its Application
  • The Chloroplast Genome and Its Application
  • The Chloroplast Genome and Its Application

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Embodiment Construction

[0034] The present invention will be described in detail through specific examples below. These embodiments are provided for a more thorough understanding of the present invention and to fully convey the scope of the present invention to those skilled in the art.

[0035] "Includes" or "comprises" mentioned throughout the specification and claims is an open term, so it should be interpreted as "including but not limited to". The subsequent description in the specification is a preferred implementation mode for implementing the present invention, but the description is for the purpose of the general principles of the specification, and is not intended to limit the scope of the present invention. The scope of protection of the present invention should be defined by the appended claims.

[0036] Unless otherwise specified, various reagents and materials used in the present invention can be purchased from the market.

[0037] 1. Extract the total DNA of the sample

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Abstract

The present invention firstly discloses the chloroplast genome of Prunus japonica, applies it to the phylogenetic research of Prunus japonica, and analyzes the phylogenetic position of Prunus japonica in the genus Prunus. In this study, molecular systematics and phylogenetic genomics methods were used to establish the phylogenetic relationship of the species of the subgenus Sakura through the chloroplast genome. On this basis, the whole genome of the chloroplast was used as a super DNA barcode of the subgenus Sakura to identify the species of Sakura , providing technical support for the protection of cherry germplasm resources and species identification, and is of great significance for the breeding of typical cherry subgenus plants and the evaluation, protection and utilization of genetic diversity.

Description

technical field [0001] The invention belongs to the field of gene technology, and in particular relates to the chloroplast genome of rosa japonica leaf and its application. Background technique [0002] With the development of society, human beings have increasingly urgent needs for the exploration of biodiversity, the rational use of biological resources and the rapid identification of species, and DNA barcoding technology has emerged as the times require. Originally, a DNA barcode referred to a standard DNA sequence used for species identification. The father of DNA barcoding, Hebert et al., successfully identified 200 species of Lepidoptera insects using a fragment of the mitochondrial cytochrome oxidase subunit 1 (cytochromeoxidase I, COI) gene of about 680 bp, and proposed DNA fragments for the first time as distinguishing species. Similar to the concept of DNA barcodes that distinguish goods by barcodes. Each species has a DNA sequence that distinguishes it from othe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C12Q1/6895G16B30/00
CPCC07K14/415C12Q1/6895G16B30/00
Inventor 沈鑫蒋冬月柳新红
Owner ZHEJIANG FORESTRY ACAD
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