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Environment-friendly dewaxing transparent reagent as well as preparation method and application thereof

The technology of a clearing agent and clearing agent is applied in the field of environmentally friendly dewaxing clearing agent and its preparation, which can solve problems such as high temperature and achieve the effects of clear solution, low volatility and stable solution.

Pending Publication Date: 2022-04-08
GUANGZHOU LBP MEDICINE SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] In addition, paraffin itself can also be used as a dewaxing reagent. The more effective solution is to dewax the reagent after mixing the ratio of mineral oil and isopropanol. However, this method requires a higher temperature to ensure complete dewaxing

Method used

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  • Environment-friendly dewaxing transparent reagent as well as preparation method and application thereof
  • Environment-friendly dewaxing transparent reagent as well as preparation method and application thereof
  • Environment-friendly dewaxing transparent reagent as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0074] This embodiment provides an environmentally friendly dewaxing transparent reagent, which includes by parts by weight:

[0075] 1 part of n-hexadecane, 10 parts of isododecane, 1 part of tert-butanol and 32 # 1.2 parts of white oil.

[0076] The preparation method of described environment-friendly dewaxing transparent reagent comprises the steps:

[0077] Take 1 part of n-hexadecane, 10 parts of isododecane, 1 part of tert-butanol and 32 # 1.2 parts of white oil, electric stirring was carried out at 20°C for 10 min at a rotation speed of 400 rpm to obtain the environmentally friendly dewaxing transparent reagent.

Embodiment 2

[0079] This embodiment provides an environmentally friendly dewaxing transparent reagent, which includes by parts by weight:

[0080] 1 part of n-dodecane, 10 parts of iso-hexadecane, 1 part of benzyl benzoate and 32 # 1.2 parts of white oil.

[0081] The preparation method of described environment-friendly dewaxing transparent reagent comprises the steps:

[0082] 1 part of n-dodecane, 10 parts of iso-hexadecane, 1 part of benzyl benzoate and 32 # 1.2 parts of white oil, electric stirring was carried out at 25° C. for 20 minutes at a rotation speed of 300 rpm to obtain the environmentally friendly dewaxing transparent reagent.

Embodiment 3

[0084] This embodiment provides an environmentally friendly dewaxing transparent reagent, which includes by parts by weight:

[0085] 3 parts of n-decane, 15 parts of iso-undecane, 1 part of benzyl benzoate and 32 parts # 1.2 parts of white oil.

[0086] The preparation method of described environment-friendly dewaxing transparent reagent comprises the steps:

[0087] Take 3 parts of n-decane, 15 parts of iso-undecane, 1 part of benzyl benzoate and 32 parts # 1.2 parts of white oil were magnetically stirred at 24° C. for 15 minutes at a rotation speed of 200 rpm to obtain the environmentally friendly dewaxing transparent reagent.

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Abstract

The invention provides an environment-friendly dewaxing transparent reagent as well as a preparation method and application thereof. The environment-friendly dewaxing transparent reagent comprises an initiator, a wax dissolving agent, a transparent agent and a solution modifier, the initiator comprises normal paraffin; the wax dissolving agent comprises isoparaffin; the transparent agent comprises any one or a combination of at least two of tert-butyl alcohol, PEG (Polyethylene Glycol) methacrylate, N, N, N, N-tetra (2-hydroxypropyl) ethylenediamine, benzyl benzoate, polyethylene glycol, benzyl alcohol or diphenyl ether. The environment-friendly dewaxing transparent reagent is used in the slide preparation process, the environment-friendly dewaxing transparent reagent is stable in wax dissolution, high in wax dissolution speed, low in volatility, low in odor and high in ignition point, and the tissue refractive index can be reduced, so that a slice is more uniform and transparent, and the structure of the slice is clear. The raw materials of the dewaxing transparent reagent are nontoxic and safe products, and the dewaxing transparent reagent is safe and convenient to use.

Description

technical field [0001] The invention belongs to the technical field of pathological tissue slices, and in particular relates to an environment-friendly dewaxing transparent reagent and a preparation method and application thereof. Background technique [0002] Routine paraffin-embedded tissue section (routine section) is the most widely used technique in routine histological preparation techniques. It is often used in pathology to study, observe and judge the morphological changes of cells and tissues. The combination of conventional section and immunological techniques can be used For the qualitative and positioning observation of macromolecular substances such as polypeptides and proteins in tissue slices, the combination of conventional slices and Fluorescence in situ hybridization (FISH) can be used for gene positioning and status analysis in cells. applied to research in a variety of disciplines. [0003] Formalin-fixed paraffin-embedded tissue is the most commonly use...

Claims

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Application Information

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IPC IPC(8): G01N1/28
Inventor 黄颖何瑰张会清陈绍宇
Owner GUANGZHOU LBP MEDICINE SCI & TECH
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