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Method for efficiently extracting gutta-percha

A high-efficiency technology of eucommia gum, which is applied in the direction of isolating microorganisms and adding compounds to stimulate growth, etc., can solve the problems of not mastering the optimal culture conditions of eucommia gum strains, not being able to exert the best performance of the strains, and not being able to fully measure the strains, etc. Achieve convenient screening and confirmation, improve assay efficiency, and increase amplification multiples

Pending Publication Date: 2022-04-08
HENAN BUSINESS SCI RES INST +1
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0005] Aiming at the deficiencies in the prior art, the present invention provides a method for efficiently extracting eucommia gum, which solves the problem of not being able to fully measure the effective bacterial strains decomposed and extracted from eucommia gum, and does not master the optimal culture conditions for strains that are more efficient when extracting eucommia gum. Therefore, the problem that the best performance of the strain cannot be exerted

Method used

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  • Method for efficiently extracting gutta-percha

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Embodiment 1

[0032] refer to figure 1 , a method for efficiently extracting eucommia gum, comprising the following steps:

[0033] S1: Soil sampling, collecting the humus soil under the eucommia forest, and then set aside;

[0034] S2: Enrichment culture, adding the soil from Eucommia ulmoides forest to the culture medium to enrich bacteria, thermophiles and fungi. By enriching the soil, more colonies can be obtained, which is convenient for later culture experiments;

[0035] S3: Separation by coating. After the enrichment, take 100 μL of the bacterial liquid and use the plate coating method to separate the bacteria. Pick the strains with fast growth rate, dominant colony number, different color and shape, and carry out the isolation, purification and preservation of the strains. Separation of bacteria by coating method, and then picking the bacteria with fast growth rate and dominant number of colonies for inoculation can quickly separate the colonies, which is convenient for subsequent...

Embodiment 2

[0044] refer to figure 1 , a method for efficiently extracting eucommia gum, comprising the following steps:

[0045] S1: Soil sampling, collecting the humus soil under the eucommia forest, and then set aside;

[0046] S2: Enrichment culture, adding the soil from Eucommia ulmoides forest to the culture medium to enrich bacteria, thermophiles and fungi. By enriching the soil, more colonies can be obtained, which is convenient for later culture experiments;

[0047] S3: Separation by coating. After the enrichment, take 100 μL of the bacterial liquid and use the plate coating method to separate the bacteria. Pick the strains with fast growth rate, dominant colony number, different color and shape, and carry out the isolation, purification and preservation of the strains. Separation of bacteria by coating method, and then picking the bacteria with fast growth rate and dominant number of colonies for inoculation can quickly separate the colonies, which is convenient for subsequent...

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Abstract

The invention relates to the technical field of gutta-percha extraction, and discloses a method for efficiently extracting gutta-percha, which comprises the following steps: S1, soil sampling: collecting humus soil under a eucommia ulmoides forest for later use; s2, enrichment culture: adding soil taken from the eucommia ulmoides forest into a culture medium to enrich bacteria, thermophilic bacteria and fungi; s3, coating separation: after enrichment is finished, taking 100 microliters of bacterial liquid, and separating bacteria by using a plate coating method; s4, activation culture: the separated and purified strains are put into an activation culture medium for activation culture, and the activation culture medium comprises a basic culture medium and an activation component; and S5, determining the degradation rate. According to the method, real-time detection can be carried out, the detection efficiency can be improved, the detection time and temperature can be controlled, the determination efficiency can be improved, and the optimal efficiency of gutta-percha extracted by strains can be detected by optimizing enzyme activity conditions, so that the extraction effect of the gutta-percha is improved.

Description

technical field [0001] The invention relates to the technical field of eucommia gum extraction, in particular to a method for efficiently extracting eucommia gum. Background technique [0002] Eucommia ulmoides gum is a kind of polymer compound existing in Eucommia ulmoides. The bark, leaves and seed coat of Eucommia ulmoides are rich in eucommia ulmoides gum. Seeds contain 10% to 18%. Eucommia gum, as a natural polymer material, is internationally recognized as a glue source that can replace traditional clover rubber. It has good insulation, acid and alkali resistance, corrosion resistance, and abrasion resistance. It can be vulcanized to produce high elastomers. It can also control the degree of cross-linking to produce new materials, which have huge market application prospects and value in the fields of industry, national defense, transportation, aerospace, medical treatment, etc. Therefore, Eucommia gum is a high-quality natural rubber resource with great development p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J3/00C08L7/00C12N1/02C12N1/38
Inventor 朱海华王晓瑞王鋆坦平洋张梦雪郭碧珊谭静王法云
Owner HENAN BUSINESS SCI RES INST
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