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Method for detecting blood glucose concentration in real time in in-vitro blood glucose degradation process

A technology for blood glucose and blood sugar concentration, which is applied in the field of real-time detection of blood glucose concentration, can solve the problems of blood glucose concentration lower than the target range, cumbersome operation, time-consuming and laborious, etc., and achieve real-time detection of blood glucose concentration, accelerated glucose degradation, and less interference factors.

Pending Publication Date: 2022-03-25
江苏鱼跃凯立特生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] When making isolated blood samples with different blood sugar concentrations, when the blood sugar concentration is lower than the target range, the blood sugar concentration is increased by adding glucose solution to the blood sample; When the blood sugar concentration is high, only the method of standing at room temperature can be adopted to degrade the glucose in the blood sample, and the blood sugar concentration will automatically decrease, and the blood sugar concentration of the blood sample will be detected after a period of time.
This method of detecting the blood sugar concentration at multiple intervals during the glucose degradation process is not only cumbersome, time-consuming and laborious to operate, but also often causes the blood sugar concentration to fall below the target range. Instead, glucose solution needs to be added to increase the blood sugar concentration. In order to reach the target range, there are May need repeated adjustments

Method used

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  • Method for detecting blood glucose concentration in real time in in-vitro blood glucose degradation process
  • Method for detecting blood glucose concentration in real time in in-vitro blood glucose degradation process
  • Method for detecting blood glucose concentration in real time in in-vitro blood glucose degradation process

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Embodiment 1

[0056] A method for real-time detection of blood glucose concentration during the degradation of glucose in isolated blood, said detection method comprising the following steps:

[0057] Step 1. Obtain the hematocrit HCT parameter in the isolated blood sample, the unit is a percentage, and then adjust the hematocrit HCT parameter to the preset range; the preset range of the hematocrit HCT parameter in this embodiment is 30% to 60% . In this embodiment, the centrifugation method is used to obtain the hematocrit HCT parameters in the isolated blood sample. The specific steps include placing the isolated blood sample in a temperature tube or capillary glass tube with uniform aperture, and after centrifugation, the isolated blood sample is layered. , calculate the volume ratio of the reduced red blood cell layer to the whole blood, and obtain the hematocrit HCT parameter. The specific steps for adjusting the hematocrit HCT parameters to the preset range are as follows: if the HCT...

Embodiment 2

[0113]A method for real-time detection of blood glucose concentration during the degradation of glucose in isolated blood. The difference between Example 2 and Example 1 is that in Example 2, the value of A is -0.8, the value of B is -24.2, and the value of C is 0.43 , the value of D is 0.2, the value of E is 14.8, and the value of F is 3.4. When the HCT parameter is selected as 30%, C 初 Set to 140mg / dL; HCT parameter selection 42%, C 初 Set to 132mg / dL; HCT parameter selection 50%, C 初 It is set to 122mg / dL; when the HCT parameter is selected as 60%, C 初 Set to 116mg / dL.

[0114] The measurement method and calculation method are the same as those in Example 1, and the data tables in Tables 6 to 9 are obtained.

[0115] Table 6 Example 2 temperature is 4 ℃ when the measured value and calculated value comparison of blood glucose concentration

[0116]

[0117] Continuing from the table

[0118]

[0119]

[0120] Continuing from the table

[0121]

[0122] Tabl...

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Abstract

A method for detecting blood glucose concentration in real time in an in-vitro blood glucose degradation process comprises the following steps: step 1, acquiring hematocrit HCT parameters in an in-vitro blood sample, and then adjusting the hematocrit HCT parameters to be within a preset range; 2, acquiring the initial blood glucose concentration C < initial > of the in-vitro blood sample treated in the step 1; and step 3, setting the temperature T of the sample chamber, with the unit being Celsius degree, and calculating the blood glucose degradation rate K through an empirical formula according to the adjusted hematocrit HCT parameter, the initial blood glucose concentration C initial and the temperature T. 4, degrading the in-vitro blood sample for a period of time t; 4 < = Tlt; when 17, 0 < = tlt; 1; 4 < = Tlt; 17, and t > = 1; 17 < = T < = 37; if T and t are in different ranges, corresponding formulas are selected to calculate the real-time blood glucose concentration C < real > of the in-vitro blood sample.

Description

technical field [0001] The invention relates to the technical field of electrochemical detection, in particular to a method for real-time detection of blood glucose concentration during the degradation process of isolated blood glucose. Background technique [0002] With the advancement of science and technology and the change of people's living habits, the field of home care has been paid more and more attention. In addition to being able to grasp the real-time status of patients at any time, it has also developed many inspection items that could only be carried out in hospitals to self-measurement at home. [0003] Among them, diabetes is a common home care disease, and blood sugar monitoring is one of the important care items for diabetic patients. Blood glucose meters currently on the market can test blood glucose with venous blood and capillary blood, among which the use of capillary blood samples is the most convenient, but clinical or laboratory internal evaluation of...

Claims

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Application Information

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IPC IPC(8): G01N27/26
CPCG01N27/26
Inventor 危亮许云琴潘雨倩张凯戎丹晶梅丹军赵梁
Owner 江苏鱼跃凯立特生物科技有限公司
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