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Bacillus velezensis DMW1 strain and application thereof

A technology of Bacillus Velez and Bacillus, applied in the field of agricultural microorganisms, can solve the problems of increasing the difficulty of preventing and controlling soil-borne diseases, the imbalance of soil micro-ecological environment, and harmful soil beneficial microorganisms, etc., to achieve the prevention and treatment of soybean Phytophthora root rot , promote crop growth, and broad-spectrum inhibitory activity

Active Publication Date: 2022-03-25
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chemical pesticides are mostly used to prevent and control such diseases, but long-term irrational use has led to the gradual emergence of the "3R" problem. Difficulty of prevention

Method used

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  • Bacillus velezensis DMW1 strain and application thereof
  • Bacillus velezensis DMW1 strain and application thereof
  • Bacillus velezensis DMW1 strain and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0036] The DMW1 strain is Bacillus velesii. A DMW1 strain was obtained by isolation from the potato field soil and face-to-face screening on a plate, and was identified as Bacillus velezensis by tandem analysis of the obtained 16S rDNA, gyrB, and tuf gene sequences.

[0037] The Bacillus velezensis DMW1 strain was preserved on August 25, 2021 in the General Microbiology Center of the China Committee for the Collection of Microbial Cultures. The preservation address is: No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, China Institute of Microbiology, Academy of Sciences, deposit number CGMCC No.23191.

[0038] PCR amplification and sequencing of 16S rRNA, gyrB and tuf genes of Bacillus velezensis DMW1 strain.

[0039]The strain was inoculated in liquid LB medium, shaken at 200 rpm, 37°C for 12 hours, collected by centrifugation, and the genomic DNA was extracted with the bacterial extraction kit from Zhuangmeng Company, and then used the forward primer 27F (5'-AGA...

Embodiment 2

[0044] The live bacteria of Bacillus velezensis DMW1 strain and its lipopeptide extracts have inhibitory activity against pathogenic oomycetes, fungi and bacteria.

[0045] The general fermentation culture method of Bacillus velezensis DMW1 strain is as follows: inoculate DMW1 strain into LB liquid culture medium and carry out a large amount of shaking culture, the temperature is 37°C, the shaker speed is 200rpm, and the fermentation time is 12 hours. The composition of LB medium is: 10g Tryptone, 10g NaCl, 5g Yeast extract, pH7.0, 1000ml distilled water.

[0046] The invention relates to a method for extracting and fermenting the active substance of Bacillus velezensis DMW1 strain.

[0047] DMW1 strain obtained by common fermentation in advance was inoculated into Landy medium at a 1% (v / v) inoculum size for shake-flask fermentation at a temperature of 37° C., a shaker speed of 200 rpm, and a fermentation time of 48 hours. The fermentation medium consists of: 5gL-Glutamicaci...

Embodiment 3

[0051] The Bacillus velezensis DMW1 strain was detected by HPLC and mass spectrometry and found that it can produce three lipopeptides: fengycin, iturin, surfactin, HPLC and MOLDI-TOF-MS analysis diagrams are attached image 3 shown.

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Abstract

The invention discloses a bacillus velezensis DMW1 strain and application thereof, the DMW1 strain is classified and named as bacillus velezensis, the DMW1 strain is preserved in China General Microbiological Culture Collection Center on August 25, 2021, and the preservation number is CGMCC No.23191. The invention further discloses a method for preparing the bacillus velezensis DMW1 strain. The strain screened by the invention has broad-spectrum inhibitory activity on crop soil-borne pathogens, and can be used as a microbial coating agent to significantly promote crop growth and prevent and treat soybean phytophthora root rot and tomato bacterial wilt.

Description

technical field [0001] The invention belongs to the technical field of agricultural microbes, and relates to a strain of Bacillus Velez DMW1 and its application, in particular to a strain of Bacillus Velez DMW1 which is isolated from a potato field and has growth-promoting and antibacterial activities. It is used as a microbial coating agent in promoting plant growth and preventing and controlling plant diseases. Background technique [0002] Soil-borne diseases seriously endanger the health of crops and soil systems, causing serious economic losses. Such as root rot caused by oomycetes Phytophthora (Phytophthora); fungi Fusarium (Fusarium) and Verticillium (Verticillium) fungi caused various crop wilts; bacteria Raueria (Ralstonia) caused by bacterial wilt of crops. Such soil-borne pathogens can reproduce in the soil and accumulate year by year, becoming a major obstacle to continuous cropping. Chemical pesticides are mostly used to prevent and control such diseases, but...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/22A01C1/06A01P1/00A01P3/00A01P21/00C12R1/07
CPCA01N63/22A01C1/06Y02A40/10
Inventor 董莎萌伍辉军王源超陈汉张雅铭
Owner NANJING AGRICULTURAL UNIVERSITY
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