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Bacterial strain resisting selenium and preparing process thereof,and application in preparing medicine of preventing and treating arsenism

An arsenic poisoning and bacterial strain technology, which is applied in fungi, pharmaceutical formulations, and medical preparations containing active ingredients, etc., can solve the problem of difficult operation of drilling wells, unpredictable arsenic content in well water, and ineffective arsenic poisoning. prevention methods, etc.

Inactive Publication Date: 2004-03-17
中国科学院地理研究所
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AI Technical Summary

Problems solved by technology

Practice has proved that although the method of drinking low-arsenic water has certain effects, it cannot effectively eliminate the excess arsenic accumulated in the human body and repair damaged tissues
At the same time, in some areas, it is necessary to dig wells for water, which makes the investment in fixed equipment relatively large. Under the current economic conditions in our country, it is difficult to ensure that all regions can bear such a large economic burden; The arsenic content in water is difficult to predict, making it difficult to drill wells for water in practice
The second method is to take drugs; at present, the emergency medicine commonly used for heavy metal poisoning in my country is mercapto compound, but the toxic and side effects of mercapto compound are relatively large, and this drug cannot be used for a long time, and has no obvious repair effect on signs and damaged tissues
[0007] To sum up, there is currently no effective prevention and control method for endemic arsenic poisoning

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0078] Saccharomyces 1419, 1571, 1422, 1603, 1328 and 1599 purchased from Beijing Institute of Nutrient Sources were selected as primary strains.

[0079] The aforementioned bacterial strains were respectively inoculated in 150ml of matrix, and cultured for 2 days on a shaker at 28°C with an amplitude of 80 times / min to recover, and then the recovered bacterial strains were respectively inoculated in the 5, 50, 100, 200, 300, 400 and 500PPM on the flat medium, cultured at 30°C for 3 days, and selected 3 strains that can still grow on the medium with high selenium concentration as the double selection strains.

[0080] a. Inoculate the selected re-selected strains in 10PPM selenium flat culture medium and culture at 30°C for 3 days; then transfer to another new 10PPM same flat culture medium and continue to culture under the same conditions 3 days; then transfer to another new 10PPM same plane culture medium until the growth of the bacteria is stable and the growth is vigorous...

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Abstract

A bacteria spawn resisting high selenium is prepared through primary selection, secondary selection and naturalized culture. It has higher bioactivity and greatly reduced toxin and can normally grow in high-Se (50PPM) culture medium. Its preparing process and its application in medicines to prevent and cure arsenism are also disclosed.

Description

technical field [0001] The present invention relates to a microorganism for treating endemic diseases and its preparation method and the application of the microorganism in the medicine for preventing and treating arsenic poisoning. Specifically, the present invention relates to a high-selenium-resistant strain for treating arsenic poisoning and its preparation method And the application of the high-selenium-resistant strain in the preparation of medicines for preventing and treating arsenic poisoning. Background technique [0002] Endemic arsenicism is prevalent in many countries and is one of the common multiple diseases that seriously threaten human health. Its harm not only manifests as short-term damage to health, but also causes systemic damage to multiple physiological systems of the human body and long-term malignancy. Change - cancer, mutation and distortion, so the prevention and treatment of arsenic poisoning are highly valued by the World Health Organization and ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/16
Inventor 侯少范王五一杨林生李德珠
Owner 中国科学院地理研究所
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