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Method and performing blood cell counts

A technology of white blood cells and red blood cells, applied in the field of assessing the components in whole blood samples, can solve the problems of no guarantee, time-consuming and expensive dilution process, etc., and achieve the effect of avoiding costs and problems, eliminating purchases and avoiding the possibility of errors

Inactive Publication Date: 2004-03-10
斯蒂芬·C·沃德罗 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A disadvantage of this method of evaluating whole blood samples is that the dilution process is time consuming and expensive
Therefore, the method is limited to the use of longer wavelength fluorescence, and there is no guarantee for the detection of RBC’s and platelets or the detection of any cell morphology.

Method used

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  • Method and performing blood cell counts
  • Method and performing blood cell counts
  • Method and performing blood cell counts

Examples

Experimental program
Comparison scheme
Effect test

Embodiment I

[0036] refer to Figure 5 and 6 , WBC's 34 in anticoagulated whole blood samples admixed with a small amount of sensitive colorant can be assessed within the sample chamber 10 having a through plane approximately equal to 20 μm through the sample chamber 10 or a portion of the sample chamber 10 Thickness 20 (see Figure 2~4 ). EDTA is an example of an applicable anticoagulant, and the brightest fluorescent ultra-reactive stains (such as Acridine Orange, Basic Orange-21, etc.) are examples of applicable sensitive stains. A chamber through-plane thickness 20 of about 20 [mu]m was selected for two reasons. First, the assessment volume contains a suitable number of WBC's 34 for detection; The sample volume being evaluated is generally determined by the cross-sectional area of ​​the evaluation viewing zone 38 and the through-plane thickness 20 of the sample. As previously mentioned, several methods can be applied to optimize the exact through-plane thickness 20 of the viewing ...

Embodiment II

[0038] The method described in Example 1 can be used to assess platelets 36 in anticoagulated whole blood samples. Since platelets are present in much greater quantities than WBC's 34, a chamber region with a through-plane thickness 20 of about 5 [mu]m in size is used. Each viewing zone 38 having a one square millimeter cross-sectional area within a chamber region with a through-plane thickness 20 of 5 μm represents a sample volume of .005 μl, so a typical individual will contain about 1250 platelets 36 . Platelets can be assessed using the same brightest fluorescent hyperactive stains and methods used for WBC's34. Platelets 36 can be assessed in the same chamber 10 as used to assess WBC's 34, provided that the chamber has regions of varying through-plane thickness dimensions, such as those described above with sloped, stepped, and / or arcuate walls.

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Abstract

A method for evaluating constituents of a sample of substantially undiluted anti-coagulated whole blood is provided which includes the steps of a) providing a sample chamber; b) admixing a sensible colorant with the sample of whole blood; c) inserting the admixed sample into the sample chamber; d) quiescently holding the admixed sample for a period until rouleaux and lacunae form within the sample; and e) evaluating a target constituent disposed within the lacunae.

Description

field of invention [0001] The present invention relates to methods and devices for analyzing whole blood samples, and to methods and devices for assessing components (eg white blood cells, platelets, etc.) in whole blood samples. Background of the invention [0002] Recent advances in analytical hematology have increased the quantity and quality of information obtainable from patient blood samples. As a result, interest in the use of patient blood samples as a diagnostic tool has also increased in the medical community. However, methods for analyzing blood samples have not kept pace with the available information in every respect. Historically, blood samples were assessed by smearing a small amount of undiluted blood on a glass slide, drying, fixing and staining, and examining the smear under a microscope. Reasonable results can be obtained from this smear, but the accuracy and reliability of the data is largely dependent on the experience and skill of the technician. In ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/49A01N1/02G01N1/18G01N15/05G01N15/14G01N21/03G01N21/76G01N33/48G01N33/50G01N33/72G01N33/86
CPCG01N33/5005G01N33/5002G01N15/05Y10T436/25375
Inventor 斯蒂芬·C·沃德罗
Owner 斯蒂芬·C·沃德罗
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