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Chili rhizosphere secretion collecting device and collecting method

A collection device and exudate technology are applied in the field of collection and sampling of pepper root exudates and rhizosphere soil, and can solve the problems of gradient change of root system properties, difficulty in distinguishing rhizosphere and non-rhizosphere, and technical difficulties.

Active Publication Date: 2022-03-01
GUIZHOU INST OF BIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If you want to collect pepper rhizosphere exudates and rhizosphere soil, you need to distinguish rhizosphere soil from non-rhizosphere soil, but it is difficult to actually distinguish rhizosphere from non-rhizosphere soil, because there are gradient changes in the properties of the root system
It is technically difficult to accurately distinguish the root-soil interface and collect soil samples under normal conditions

Method used

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  • Chili rhizosphere secretion collecting device and collecting method
  • Chili rhizosphere secretion collecting device and collecting method
  • Chili rhizosphere secretion collecting device and collecting method

Examples

Experimental program
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Embodiment 1

[0039] refer to Figure 1-5 , the present embodiment provides a device for collecting capsicum rhizosphere exudates, comprising a culture bucket, wherein a culture medium and an irrigation assembly are arranged in the culture bucket;

[0040] The cultivation barrel includes a barrel body 1, the bottom end of the barrel body 1 is fixedly connected with a bottom plate 2, the bottom plate 2 is tapered and the smaller end is located in the barrel body 1, the smaller end of the bottom plate 2 is provided with a through hole 3, and the through hole 3 A filter element 12 is provided;

[0041] The culture substrate includes a first culture layer 4, the first culture layer 4 is laid in the barrel body 1 and the top of the first culture layer 4 is higher than the through hole 3, and the top of the first culture layer 4 is laid with a second culture layer 5;

[0042] The watering assembly includes irrigators 6 arranged at equal intervals in the circumferential direction in the second cu...

Embodiment 2

[0062] The difference with Embodiment 1 is that in this embodiment, the irrigator 6 is replaced by a PVC plastic pipe, the bottom end of the PVC plastic pipe is closed, and the side wall of the PVC plastic pipe is provided with some miniature water seepage holes, and the nutrient solution is added to the After entering the PVC plastic pipe, it first penetrates into the protective layer 16, and then penetrates into the second culture layer 5 through the protective layer 16.

Embodiment 3

[0064] The difference with embodiments 1 and 2 is that in the present embodiment, the filter element 12 adopts 18 meshes of nylon mesh, the sandstone particle size of the first culture layer 4 is greater than 1mm and less than 2mm, and the fibrous roots of pepper plants 17 pass through the filter element 12 Form a horizontal root surface. At this time, when collecting capsicum rhizosphere exudates, the sterile filter paper is in direct contact with the capsicum root, which makes the collection more convenient.

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Abstract

The pepper rhizosphere secretion collecting device comprises a culture barrel, and a culture medium and an irrigation assembly are arranged in the culture barrel; the culture barrel comprises a barrel body, a bottom plate is fixedly connected to the bottom end of the barrel body, the bottom plate is conical, the smaller end of the bottom plate is located in the barrel body, a through hole is formed in the smaller end of the bottom plate, and a filtering piece is arranged in the through hole; the culture medium comprises a first culture layer which is laid in the barrel body, the top end of the first culture layer is higher than the through hole, and a second culture layer is laid at the top end of the first culture layer; the irrigation assembly comprises irrigators which are circumferentially arranged in the second culture layer at equal intervals, the top ends of the irrigators extend out of the barrel body, the bottom ends of the irrigators extend into the first culture layer, and the bottom ends of the irrigators are lower than the through holes. According to the device and the method, the rhizosphere soil and the rhizosphere secretions of the peppers can be obtained while the growth of the peppers is not disturbed, and the rhizosphere secretion-absorption dynamic process of the soil-cultured peppers is dynamically monitored and analyzed at different root system positions and different plant growth periods under controllable conditions.

Description

technical field [0001] The invention relates to the collection and sampling of capsicum root exudates and rhizosphere soil in the field of biotechnology, in particular to a device and method for collecting capsicum rhizosphere exudates. Background technique [0002] During the growth of pepper, the root system not only absorbs nutrients and water from the environment, but also secretes protons, releases inorganic ions, and exudes or secretes a large amount of organic matter into the growth medium. These substances and root tissue shedding are collectively referred to as root products (root products), that is, root exudates. As early as the 18th and 19th centuries, people (plenk1795; decandolle, 1830) observed the growth-promoting and inhibiting effects of root exudates on neighboring plants. However, until the 1950s when people realized the mutually beneficial relationship between root exudates and nitrogen fixation that promotes plant growth, the research in this field bec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/04G01N1/28A01G9/02
CPCG01N1/04G01N1/28A01G9/02Y02P60/21
Inventor 张珍明黄冬福曾宪平刘盈盈张家春罗文敏吴先亮牟桂婷
Owner GUIZHOU INST OF BIOLOGY
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