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Collecting method and apparatus for secretion from root system of plant in pot

A technology for root exudates and plant roots, applied in the field of collection methods and collection devices for plant root exudates under soil culture conditions, to achieve good continuity and repeatability, improve test accuracy and recovery rate, and reduce adsorption and fixation effects

Inactive Publication Date: 2009-11-04
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are no reports about the collection method and collection device of this root exudate both at home and abroad at present.

Method used

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  • Collecting method and apparatus for secretion from root system of plant in pot

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Embodiment Construction

[0027] The present invention will be described in further detail below in conjunction with the examples, but the embodiments of the present invention are not limited thereto.

[0028] A collection device for soil plant root exudates, including a growth box and a collection box. The side of the growth box is a right-angled triangular plate, and a slot 5 parallel to the hypotenuse is provided on the right-angled triangular plate, and an inclined plexiglass insert plate 2 is fixed through the slot between the two triangular plates. An organic perforated glass plate 7 is arranged between the growth box and the collection box, and a polytetrafluoroethylene film 4 is pasted on the organic perforated glass plate 7. Two rows of small holes 20 are respectively arranged on the upper and lower sides of the organic perforated glass plate 7, and the upper and lower rows of holes 20 are arranged on the organic perforated glass plate. Aperture corresponds to the upper collection box 10, and ...

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Abstract

The invention discloses a method and a collection device for collecting root exudates of soil cultured plants. The collection device includes a growth box and a collection box, and a perforated glass plate pasted with a thin film is arranged between the growth box and the collection box; the collection holes on the upper and lower collection boxes are connected with anion and cation resin pipes through conduits. The collection method is first to allow plants to grow in the soil of the growth box, and then the plant roots pass through the film and enter into the collection box equipped with quartz sand and glass beads; The present invention is especially suitable for the research of plant root exudates under soil cultivation conditions; the root growth is not disturbed, the rhizosphere environment is not damaged, and the collection environment is closer to the natural growth state of plants; the present invention can collect the exudates of shallow roots and deep roots respectively , Good continuity and high recovery rate.

Description

technical field [0001] The invention relates to the field of collecting plant root exudates, in particular to a method and a collecting device for collecting plant root exudates under soil cultivation conditions. Background technique [0002] At present, the solution collection method is generally used to collect plant root exudates. The specific operation method is: the plant roots first grow in the nutrient solution, and then transfer to the treatment solution. After a period of treatment, the collected treatment solution is the root exudate. The root exudate collection device of Chinese patent CN2581964YHE and CN1482443A design. The solution collection method is widely used because of its simple operation and convenient analysis. However, the root exudates obtained by the solution collection method can hardly reflect the actual situation of plant root exudates under soil conditions. This is mainly due to: complex soil composition, diverse forms of nutrients, uneven dist...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/02G01N1/28
Inventor 沈宏王占义严小龙
Owner SOUTH CHINA AGRI UNIV
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