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Preparation method of autologous venous blood cell activator

A technology of blood cells and activators, applied in the field of medicine, can solve the problem that the characteristics of single factors have not been analyzed in detail, and achieve the effect of strengthening the function and effect, and prolonging the effect time.

Pending Publication Date: 2022-03-01
张江平
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AI Technical Summary

Problems solved by technology

While the value of human platelet lysates in stem cell culture is well established, the characterization of individual factors in human platelet lysates has not been analyzed in detail

Method used

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Experimental program
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Embodiment 1

[0016] A preparation method of an autologous venous blood cell activator, which is composed of a first part, a second part and a third part, the first part is a lymphocyte and stem cell separation liquid, a platelet protection agent, preventing blood autolysis and anticoagulation, centrifugal separation, filtration In addition to red blood cells; the second part is platelet activator, including calcium chloride with a final concentration of 40m / m, bovine thrombin with a final concentration of 100Uml, and a small amount of protein kinase A; add the first part and the second part to the prepared one at a ratio of 1:10 In the sample; the third part is an acidic solution, which is used to lower the pH value of the sample to 6.5-6.7, and incubate at 37° C. for 10-30 minutes.

[0017] Autologous blood cell activators can maximize the enrichment of lymphocytes, immune cells, stem cells and platelets, stimulate the production of a large number of exosomes by autologous blood cell activ...

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PUM

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Abstract

According to the preparation method of the autologous venous blood cell activator provided by the invention, blood cells are centrifuged, concentrated and separated, and on the basis of human platelet lysates, exosome adolescin with high concentration and high activity can be generated and obtained through activation of the optimized autologous venous blood cell activator, so that the autologous venous blood cell activator can be used for preparing the exosome adolescin. The protein with the cell adhesion function contains adhesion factors such as a high-concentration growth factor, an immunomodulatory factor, a cell activating factor, a cell migration factor, a cell proliferation factor, an angiogenesis factor, a cell regeneration and repair anti-aging factor, fibrin, fibronectin and vitronectin, and the cell regeneration and repair anti-aging factor. Cell signal transduction and synergistic regulatory factors, etc.

Description

technical field [0001] The present application relates to the technical field of medicine, in particular to a preparation method of an autologous venous blood cell activator. Background technique [0002] Platelet-rich plasma is called "high-concentration platelet plasma" in Chinese, and each cubic millimeter of high-concentration platelet plasma contains about one million units of platelets. In the state of high concentration, human platelets can produce proteins with cell adhesion function, and when the pH value drops from 7.0-7.2 to 6.5-6.7, adding appropriate calcium chloride can promote the secretion of a large number of platelets. Nine kinds of growth factors for wound, tissue healing and cell regeneration, so high-concentration platelet plasma is also called growth factor-rich plasma. [0003] Human platelet lysate is an alternative supplement to fetal bovine serum in human cell culture. It is commonly used to supplement the basal medium in human mesenchymal stem ce...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/17A61P17/00A61P17/18A61K8/99A61Q19/02A61Q19/08A61K35/19
CPCA61K35/19A61K35/17A61P17/00A61P17/18A61K8/99A61Q19/02A61Q19/08A61K2300/00
Inventor 张江平
Owner 张江平
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