Application of mir-671-5p reagent for detection of human serum exosomes and osteoporosis detection kit

Abstract

The present invention provides an application of detecting miR-671-5p reagent in human serum exosomes and an osteoporosis detection kit, which includes reverse transcription primers of miR-671-5p, fluorescent probes, and PCR primers, and can also be It further includes internal reference reverse transcription primers, fluorescent probes and PCR primers, and the kit further includes miR-671-5p reference and internal reference. The kit uses double fluorescent quantitative PCR to detect the absolute concentration of exosomal miR-671-5p from a small amount (200 μL) of serum or plasma. The detection method is specific, efficient and sensitive. The kit can be used for the prediction and evaluation of osteoporosis in the elderly, and has important clinical significance.

Description

Application of miR-671-5p reagent for detecting human serum exosomes and osteoporosis detection Reagent test kit technical field The present invention relates to biology, medical detection field, particularly detect miR-671-5p reagent in human serum exosomes application and osteoporosis detection kit. Background technique The prevalence rate of the elderly over 60 years old in my country is 36%, and there may be no obvious symptoms in the initial stage, which is called "silent disease", and it is difficult to For early detection and diagnosis, currently only bone density testing can be used for screening, prevention is better than treatment, and there is no better detection method. Exosome (exosome) is a kind of lipid bilayer structure, nano-scale vesicles secreted from the inside to the outside of the cell. The diameter is 30-150nm, containing specific proteins, lipids, nucleic acids and other substances, and various components it contains mediate the relationship...

AI Technical Summary

Problems solved by technology

[0004] Extract exosomes from serum and use fluorescent PCR to detect miR-671-5p. Currently, there are dye method and probe method. The dye method is not as specific as the probe method. At present, these two methods are relatively quantified by the internal reference Ct value The method cannot accurately quantify the specific molecular copy number, and the detection limit and sensitivity are unknown

Moreover, the current existing methods are all single-channel single-color detection, and microRNA and internal reference each need a separate tube for PCR, which results in low detection efficiency and high cost.

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