Micro-fluidic chip and method for realizing rapid and accurate cell sorting at low flow rate

Abstract

The invention discloses a micro-fluidic chip and method for realizing rapid and accurate cell sorting at a low flow rate. The micro-fluidic chip comprises a micro-fluidic chip flow channel layer, wherein the micro-fluidic chip flow channel layer comprises a sample flow double-separation inlet flow channel, a sheath flow inlet flow channel, an extrusion straight flow channel, a contraction and expansion array flow channel, a sorting straight flow channel and a sudden expansion sorting flow channel, the viscoelastic sheath flow and the viscoelastic sample flow both adopt viscoelastic solutions, cells are transversally migrated to different degrees in the contraction and expansion array flow channel according to the sizes of the cells, rapid sorting in a simple flow channel structure is realized, and meanwhile, a certain protection effect on vulnerable cells is achieved as the sorting is carried out at a low flow rate.

Description

technical field [0001] The present invention relates to the field of biological particle manipulation based on microfluidic chips, and specifically relates to a method of integrating viscoelastic straight channel wall and channel center double-balanced position focusing technology and contraction and expansion array channel sorting technology to realize cell size-based sorting Microfluidic chips and methods. Background technique [0002] At present, the detection of rare cells such as circulating tumor cells (CTCs) has been widely used in the early diagnosis, prognosis evaluation, curative effect detection and personalized treatment of patients with malignant tumors. Research hotspots. [0003] With the advantages of small sample volume, low cost, simple operation, rapid detection and high precision, microfluidic chips have great development potential and broad application prospects in the detection and sorting of rare cells. At present, in the microfluidic chip of inertia...

AI Technical Summary

Problems solved by technology

In order to achieve effective cell sorting, the common Newtonian fluid inertial microfluidic technology is usually carried out at a higher flow rate, and the sorting efficiency is higher, but the manipulation accuracy for small-sized cells is limited, and it is not friendly to fragile cells

In the inertial straight channel, the flow rate of the viscoelastic fluid is low, and the efficiency of cell manipulation is limited to a certain extent, but the particle has a higher discrimination accuracy.

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