Functional fermented feed for laying hens as well as preparation method and application thereof
A fermented feed and functional technology, applied in the field of functional laying hen fermented feed and its preparation, can solve the problems that probiotics cannot have strong antagonism, the quality of fermented feed is uneven, and the storage time is increased, so as to improve the feed quality. The shelf life, high strain richness, and the effect of improving production performance
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Embodiment 1
[0041] This embodiment provides a functional fermented feed for laying hens, with reference to figure 1 , its preparation method comprises the following steps:
[0042] 1. Preparation of compound lactic acid bacteria liquid: use the improved MRS liquid medium to inoculate 0.1% of 1×10 11 cfu / g live Lactobacillus plantarum powder, 0.1% ratio of 1×10 11 cfu / g live Enterococcus faecalis powder, 0.1% ratio of 1×10 11 cfu / g live bifidobacteria powder, so that the liveness of the three lactic acid bacteria in the improved MRS liquid medium is all 10 8 cfu / mL, culture at 35°C for 20 hours, use 3mol / L NaOH to control the pH between 5.2-6.0 in the middle process, and the total number of viable bacteria can be obtained at 2×10 10 cfu / mL of lactic acid bacteria culture solution. The formula of MRS liquid modified medium is (g / L): 40.00 molasses, 10.00 peptone, 5.00 yeast extract, 10.00 beef extract powder, 1.00 diammonium hydrogen citrate, 1.00 dipotassium hydrogen phosphate, 3.00 an...
Embodiment 2
[0053] This embodiment provides a functional fermented feed for laying hens, with reference to figure 1 , its preparation method comprises the following steps:
[0054] 1. Preparation of compound lactic acid bacteria liquid: use the improved MRS liquid medium to inoculate 0.1% of 1×10 11 cfu / g live Lactobacillus plantarum powder, 0.1% ratio of 1×10 11 cfu / g live Enterococcus faecalis powder, 0.1% ratio of 1×10 11 cfu / g live bifidobacteria powder, so that the liveness of the three lactic acid bacteria in the improved MRS liquid medium is all 10 8 cfu / mL, static culture at 37°C for 16 hours, use 3mol / L NaOH to control the pH between 5.2-6.0 in the middle process, and the total number of viable bacteria can be obtained at 2×10 10 cfu / mL of lactic acid bacteria culture solution. The formula of MRS liquid modified medium is (g / L): 40.00 molasses, 10.00 peptone, 5.00 yeast extract, 10.00 beef extract powder, 1.00 diammonium hydrogen citrate, 1.00 dipotassium hydrogen phosphate, ...
Embodiment 3
[0066] This embodiment provides a functional fermented feed for laying hens, with reference to figure 1 , its preparation method comprises the following steps:
[0067] 1. Preparation of compound lactic acid bacteria liquid: use the improved MRS liquid medium to inoculate 0.1% of 1×10 11 cfu / g live Lactobacillus plantarum powder, 0.1% ratio × 10 11 cfu / g bacteria live Enterococcus faecalis powder, 0.1% ratio × 10 11 cfu / g live bifidobacteria powder, so that the liveness of the three lactic acid bacteria in the improved MRS liquid medium is all 10 8 cfu / mL, culture at 36°C for 18 hours, use 3mol / L NaOH to control the pH between 5.2-6.0 in the middle process, and the total number of viable bacteria can be obtained at 2×10 10 cfu / mL of lactic acid bacteria culture solution. The formula of MRS liquid modified medium is (g / L): 40.00 molasses, 10.00 peptone, 5.00 yeast extract, 10.00 beef extract powder, 1.00 diammonium hydrogen citrate, 1.00 dipotassium hydrogen phosphate, 3.00...
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