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Glutamic acid bacillus GH202103 strain for degrading alpha-solanine as well as preparation method and application of glutamic acid bacillus GH202103 strain

A glutamic acid bacteria and strain technology, applied in the field of microorganisms, can solve the problems of strong teratogenicity and reproductive toxicity, damage to gastrointestinal cell membranes, loss of consciousness, etc., and achieve the effects of strong degradation, easy cultivation and reproduction, and strong degradation ability.

Active Publication Date: 2022-02-08
YUNNAN AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In addition, α-solanine has high toxicity to humans and animals, and shows significant cytotoxicity to normal human liver cells. It can also inhibit the activity of cholinesterase in the human central nervous system and damage the gastrointestinal cell membrane. Eating solanine by mistake can cause Nausea, vomiting, diarrhea, loss of consciousness, paralysis, shock and other symptoms, if the rescue is not timely, it will cause death
In addition, solanine has strong teratogenic and reproductive toxicity

Method used

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  • Glutamic acid bacillus GH202103 strain for degrading alpha-solanine as well as preparation method and application of glutamic acid bacillus GH202103 strain
  • Glutamic acid bacillus GH202103 strain for degrading alpha-solanine as well as preparation method and application of glutamic acid bacillus GH202103 strain

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Embodiment 1

[0025] A glutamicibacter GH202103 strain for degrading α-solanine of the present invention, the strain is glutamicibacter, and the preserved name is Glutamicibacter halophytocola; it is preserved in the General Microorganism Center of China Microbiological Culture Preservation Management Committee, preserved The address is No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing; date of deposit: May 31, 2021; deposit number: CGMCC No.22638.

[0026] The 16S rDNA gene sequence of the α-solanine-degrading glutamicum GH202103 strain is the nucleotide sequence shown in SEQ ID No.1.

[0027] The strain was purified by streaking on LB plate and it was found that the colony of strain S was irregular, fan-shaped at the edge, light yellow, wrinkled, moist, and highly convex. Gram-stained purple, Gram-positive bacteria, cell morphology is rod-shaped.

[0028] The bacterial agent of the glutamicum GH202103 strain prepared by the glutamicum GH202103 strain of the present inventi...

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Abstract

The invention discloses a glutamic acid bacillus GH202103 strain for degrading alpha-solanine as well as a preparation method and application of glutamic acid bacillus GH202103 strain. The glutamic acid bacillus GH202103 strain for degrading alpha-solanine is GH202103, the preservation name is Glutamicibacter halophytocolaoGH202103, the strain is preserved in China General Microbiological Culture Collection Center (CGMCC), and the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing; the preservation date is May 31, 2021; the preservation number is CGMCC No.NO22638. The strain provided by the invention is a bacterium capable of degrading alpha-solanine, has the characteristics of strong degradation effect on alpha-solanine and safety to environment, and can be used for biodegradation of alpha-solanine and food safety.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a glutamicum GH202103 strain degrading α-solanine and its preparation method and application. Background technique [0002] α-solanine is a toxic glycoalkaloid present in Solanaceae crops, also known as solanine, solanine, potato toxin, etc. α-solanine has a high content in potatoes, and α-solanine in potatoes Alkaline content accounts for more than 95% of the total potato glycoalkaloids, and there are certain differences in the content of α-solanine in different tissues. α-solanine has anti-microbial, anti-insect and anti-fungal effects, and α-solanine in potato has insecticidal activity. In addition, α-solanine has high toxicity to humans and animals, and shows significant cytotoxicity to normal human liver cells. It can also inhibit the activity of cholinesterase in the human central nervous system and damage the gastrointestinal cell membrane. Eating solanine by mista...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23L5/20C12R1/01
CPCC12N1/20A23L5/28
Inventor 陈斌王文倩肖关丽杜广祖叶举辉杨宝云薛锐张栩孙淦琳付玉飞
Owner YUNNAN AGRICULTURAL UNIVERSITY
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