SNP molecular marker related to length of towel gourd and application of SNP molecular marker

A technology of molecular markers and loofah, which is applied in the direction of recombinant DNA technology, microbial measurement/testing, DNA/RNA fragments, etc., can solve problems affecting the breeding of loofah

Active Publication Date: 2022-01-25
INST OF VEGETABLES GUANGDONG PROV ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, complex environmental factors and genetic variation affect the selection of loofah varieties with different lengths

Method used

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  • SNP molecular marker related to length of towel gourd and application of SNP molecular marker
  • SNP molecular marker related to length of towel gourd and application of SNP molecular marker
  • SNP molecular marker related to length of towel gourd and application of SNP molecular marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0097] Example 1 Positioning of genes related to loofah

[0098] 1, experimental materials

[0099] Short Sweater 93075 comes from: Self-study system selected by the Vegetable Research Institute of Guangdong Academy of Agricultural Sciences.

[0100] Long rosum WJ55 comes from: a loofah self-study system breeding in the Vegetable Research Institute of Guangdong Academy of Agricultural Sciences.

[0101] The traits of the above short rumps 93075 and long rumps wj55 are like figure 1 Indicated.

[0102] 2, experimental steps

[0103] 2.1 Hybridize the short rumps 93075 and long rumps WJ55, hybridize to obtain F1, and then obtain F2, it will be planted in the greenhouse, 50 days later, investigating each single melon, and each single plant investigates 3 merchase, take average. Such as figure 2 As shown, the length of the loofah fruit is a normal distribution, indicating that the melon is a typical quantitative shape, which is a polymeric system control.

[0104] 2.2 Using the F2 gro...

Embodiment 2

[0111] Example 2 M4962 Molecular Marker Application as a Longular Molecular Molecular Molecular Molecular

[0112] 1, M4962 molecular markers and / or M4962 primer groups for identification or auxiliary identification

[0113]Planted 35 loofah products in Guangzhou Baiyun District Zhongtan Baiyun Base, normal water fertilizer management, after 50 days, investigating each loofah variety (as shown in Table 3). The M4962 primer group was used to measure the 35 pumpkin products: extract the loofah genome DNA, using the genomic DNA as a template, using the M4962 primer group for PCR amplification, and the amplification reaction system is shown in Table 1, the reaction conditions are shown in Table 2. As shown, after the PCR is completed, use the Tecan Infinite M1000 enzyme instrument to read the fluorescent signal, then use the online software snpdecoder (http: / / www.snpway.com / snpdecoder / ) to resolve the conversion fluorescent signal to get a clear and intuitive division map. And accor...

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Abstract

The invention relates to the technical field of biological molecular markers, and discloses an SNP molecular marker related to the length of a towel gourd and application of the SNP molecular marker. The SNP molecular marker is located at the 151th site of a nucleotide sequence shown in SEQ ID NO.1, and the polymorphism is A/G. The molecular marker is remarkably related to the length character of the towel gourd and can be used for identifying or assisting in identifying the length character of the towel gourd to be detected, identifying or assisting in identifying whether the fruit of the towel gourd to be detected is a long gourd or a short gourd, breeding the towel gourd with the fruit being the long gourd, breeding the towel gourd with the fruit being the short gourd and breeding the towel gourd.

Description

Technical field [0001] The present invention belongs to the field of biomolecule labeling, and specifically, the present invention relates to SNP molecules and their applications associated with loofah. Background technique [0002] Loofah is an important multi-functional vegetable. Loofah fruit is rich in vitamins, amino acids, trace elements and antioxidants, with heat-saving heat, stop hemostasis, is a multi-functional vegetables that uses a medicinal food. In recent years, as people pay attention to nutrition and health, the sowing area of ​​loofah has increased year by year, and it has become an important daily consumption vegetable. [0003] The length of the fruit fruit of loofah is an important appearance quality. Due to the different consumption habits and uses, the requirements for loofah lengths are different. In response to the consumption habits of different regions, cultivating the varieties of varieties of melons can significantly increase farmers' benefits. Howeve...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 赵钢军吴海滨罗剑宁龚浩李俊星郑晓明刘小茜
Owner INST OF VEGETABLES GUANGDONG PROV ACAD OF AGRI SCI
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