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Blueberry tissue culture seedling substrate and preparation method thereof

A technology of tissue culture seedlings and substrates, applied in botany equipment and methods, gardening methods, gardening tools/equipment, etc., can solve the problems of single function, improve regeneration efficiency, prevent tissue browning and death, and reduce impact

Pending Publication Date: 2022-01-18
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] For the basic medium of blueberry tissue culture explants, MS medium and WPM medium are usually used as basic medium, but the functions of the two are relatively single, and they can only maintain the basic survival of plants. A medium that provides good conditions for plant growth is necessary

Method used

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  • Blueberry tissue culture seedling substrate and preparation method thereof

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Effect test

Embodiment 1

[0019] A blueberry tissue culture seedling matrix comprises the following components in the following mass parts: 100 parts of MS medium, 1 part of sucrose, 0.5 part of agar, 1 part of hormone, 5 parts of coconut milk and 1 part of activated carbon. The hormones are 2,4-dichlorophenoxyacetic acid, naphthaleneacetic acid, zeatin, 2-prenylpurine and gibberellin.

[0020] The preparation method of the blueberry tissue culture seedling matrix is ​​as follows: take four kinds of mother solutions of MS medium, including macroelements, trace elements, iron salts, and organic substances, add water to the macroelement mother liquor to dilute it by 20 times, and add water to the microelement mother liquor to dilute it by 100 times , adding water to the iron salt mother liquor to dilute 200 times, and adding water to the organic substance mother liquor to dilute 100 times; pour the 4 kinds of mother liquors into the container according to the order of macroelements, trace elements, iron s...

Embodiment 2

[0023] A blueberry tissue culture seedling transplanting mechanism, including 25 parts of MS medium, 75 parts of WPM medium, 2 parts of sucrose, 0.6 parts of agar, 3 parts of hormone, 4 parts of coconut milk, and 1 part of activated carbon. The hormones are 2,4-dichlorophenoxyacetic acid, naphthaleneacetic acid, zeatin, 2-prenylpurine and gibberellin.

[0024] The preparation method of the blueberry tissue culture seedling matrix is ​​as follows: take four kinds of mother solutions of MS medium, including macroelements, trace elements, iron salts, and organic substances, add water to the macroelement mother liquor to dilute it by 20 times, and add water to the microelement mother liquor to dilute it by 100 times , adding water to the iron salt mother liquor to dilute 200 times, and adding water to the organic substance mother liquor to dilute 100 times; pour the 4 kinds of mother liquors into the container according to the order of macroelements, trace elements, iron salts, and...

Embodiment 3

[0028] A matrix for blueberry tissue culture seedlings, comprising 50 parts of MS medium, 50 parts of WPM medium, 3 parts of sucrose, 0.65 part of agar, 4 parts of hormone, 3 parts of coconut milk and 0.7 part of activated carbon. The hormones are 2,4-dichlorophenoxyacetic acid, naphthaleneacetic acid, zeatin, 2-prenylpurine and gibberellin.

[0029] The preparation method of the blueberry tissue culture seedling matrix is ​​as follows: take four kinds of mother liquors of MS culture medium, including macroelements, trace elements, iron salts, and organic substances, add water to the macroelement mother liquor to dilute it by 20 times, and add water to the microelement mother liquor to dilute it by 100 times , adding water to the iron salt mother liquor to dilute 200 times, and adding water to the organic substance mother liquor to dilute 100 times; pour the four kinds of mother liquors into the container according to the order of macroelements, trace elements, iron salts, and ...

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Abstract

The invention discloses a blueberry tissue culture seedling substrate and a preparation method thereof, and relates to the technical field of blueberry tissue culture seedling culture. Each unit of the substrate is prepared from the following components in parts by mass: 0-100 parts of an MS culture medium, 0-100 parts of a WPM culture medium, 1-5 parts of cane sugar, 0.5-0.8 part of agar, 1-10 parts of hormone, 1-5 parts of coconut milk and 0.5-1 part of activated carbon. The MS culture medium and the WPM culture medium are combined to serve as a basic culture medium, the basic requirements of plant growth are met, and the regeneration efficiency of tissue culture seedlings is improved; various substances such as hormone are added into the culture medium, so that the growth requirements of plants are adjusted, and some necessary micronutrients and physiological activators are provided; and the addition of the activated carbon is beneficial to reducing the influence of some harmful substances and preventing tissue browning death caused by phenolic substance pollution.

Description

technical field [0001] The invention relates to the technical field of transplanting blueberry tissue culture seedlings, in particular to a blueberry tissue culture seedling matrix and a preparation method thereof. Background technique [0002] Blueberry is a small berry of the Rhododendronaceae Vaccinium shrub, and is listed as one of the top five healthy foods by the International Food and Agriculture Organization. With the gradual expansion of the scale of blueberry cultivation in my country, the demand for high-quality blueberry seedlings has also increased sharply. Therefore, tissue culture of blueberries has been widely used in production practice. [0003] Plant tissue culture is a technology in which plant cells, tissues, living organs and protoplasts are inoculated on the corresponding medium under sterile operating conditions, and the culture conditions are controlled manually to obtain regenerated plants. It has the advantages of short cultivation period, fast re...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/002
Inventor 张丙秀张丙芳王振
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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