Detection kit and detection method for pathogen nucleic acid under airtight condition

Abstract

The invention discloses a detection kit and a detection method for pathogenic nucleic acid under an airtight condition, and belongs to the technical field of nucleic acid extraction. The detection kit comprises a sealing box body and a sealing cover, a first detergent chamber, a cracking buffering agent chamber, a second detergent chamber, an elution buffering agent chamber, a purification membrane chamber, a waste liquid chamber, a reaction detection chamber and a sealing membrane which is arranged on the channel and can be opened are sequentially arranged in the sealing box body from top to bottom, wherein the first detergent chamber, the cracking buffering agent chamber, the second detergent chamber, the elution buffering agent chamber, the purification membrane chamber, the waste liquid chamber and the reaction detection chamber are connected through the channel; and the detection method comprises the steps that the cracking buffering agent chamber, the first detergent chamber, the second detergent chamber and the elution buffering agent chamber are isolated from the channel through sealing films, after corresponding steps are completed, laser breaks through the sealing films, and liquid in corresponding cavities is centrifugally driven to flow into the channel. Preloaded reagents are isolated through the sealing films, laser drilling and centrifugal force driving are completely achieved in a non-contact mode, and on the basis of the chip structure design of fluid properties, pathogenic nucleic acid extraction and real-time fluorescent PCR amplification detection of the detection kit under the airtight and full-closed condition are achieved.

Description

Technical field [0001] The present invention relates to the field of nucleic acid extraction techniques, in particular, a detection kit and detection method of the protected nucleic acid under airtight conditions. Background technique [0002] Nucleic acid analysis technology, as a strong molecular diagnostic technology, it has an important application in pathogen diagnosis, infectious disease prevention, disease monitoring and treatment. The nucleic acid analysis is highly specific, high sensitivity, and the like. Since most target genes have low concentration, it is difficult to directly analyze the detection, so nucleic acid analysis methods such as nucleic acid amplification principle become the main means of current nucleic acid analysis. The complete process usually includes nucleic acid extraction, nucleic acid amplification, and three major circles of nucleic acid detection. However, the traditional nucleic acid analysis has time consuming, high cost, large workload, and ...

AI Technical Summary

Problems solved by technology

It can be seen that the production process of magnetic beads that adsorb nucleic acid cannot achieve "no nucleic acid pollution". This nucleic acid extraction and purification method is not suitable for highly sensitive virus nucleic acid detection, and there is a risk of false positives

[0008] 4. Fluorescent PCR chamber C5 adopts constant temperature hot plate PCR reaction technology, which has low sensitivity

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