Preparation method of drug-loaded vegetable protein nanoparticles

Abstract

The invention discloses a preparation method of drug-loaded vegetable protein nanoparticles. The preparation method comprises the following steps: dissolving vegetable protein and a natural hydrophobic drug to form a solution; preparing a sodium citrate-citric acid or phosphate buffer solution; respectively filling two injectors with the solution of vegetable protein and the natural hydrophobic drug, and fixing the injectors on the same injection pump; subpacking the buffer solution in the two injectors, and fixing the two injectors on the same injection pump; introducing the solution in the four injectors into a multi-channel vortex mixer through the injection pump, and receiving a product at an outlet of the multi-channel vortex mixer; and dialyzing and purifying the obtained product in ultrapure water by using a dialysis bag to obtain the spherical nanoparticles which take the vegetable protein as the carrier to wrap the natural hydrophobic drug. The invention also discloses the drug-loaded vegetable protein spherical nanoparticles obtained by the method. According to the invention, continuous reaction and preparation of the spherical nanoparticles can be realized, and the problems caused by intermittent preparation and amplification effect can be effectively solved.

Description

technical field [0001] The invention belongs to the technical field of functional nanometer materials, and in particular relates to a preparation method of drug-loaded plant protein nanoparticles. Background technique [0002] Natural plant protein has many advantages as a drug-loaded nanoparticle, such as a wide range of sources, excellent biocompatibility and biodegradability, etc. Zein and soybean protein isolate are typical representatives. The chemical structures of these two plant proteins contain both hydrophilic groups and hydrophobic groups and are amphiphilic, so that they can self-assemble to form nanostructures and be used as nanocarriers. [0003] At present, the relatively mature methods for preparing plant protein nanoparticles are anti-solvent precipitation method and pH-driven method, both of which are based on thermodynamic equilibrium self-assembly to form nanoparticles. The anti-solvent precipitation method is to dissolve the vegetable protein in a mixed...

AI Technical Summary

Problems solved by technology

[0004] 1. Low drug loading and difficult to control particle size

In the above traditional preparation methods, in order to obtain plant protein nanoparticles with a stable structure, not only need to add a large amount of plant protein, the amount is large, but also need to add a second polymer as a co-stabilizer, resulting in the prepared nanoparticles The drug loading is low (usually <10%), which makes it difficult to systematically control the particle size and particle size distribution of nanoparticles;

[0005] 2. The traditional preparation method is not only time-consuming, but also batch-type preparation, which is not easy to transform into large-scale continuous production

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