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Detection method of steroid hormone

A steroid hormone and detection method technology, applied in the field of steroid hormone detection, achieves high precision and accuracy

Active Publication Date: 2021-12-31
广西壮族自治区海洋环境监测中心站
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few studies and reports on the existence of steroid hormones in seawater, marine sediments, and organisms in sea areas.

Method used

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  • Detection method of steroid hormone
  • Detection method of steroid hormone

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A detection method for steroid hormones, comprising the following steps:

[0035](1) Sample pretreatment: 0.75g of seawater sediment (taken from the coastal waters of Guangxi) was added to 25mL of solvent for ultrasonic extraction, wherein the solvent was methanol, ethylene glycol derivatives and acetic acid with a volume ratio of 1:0.5:1 Ethyl ester, then ultrasonically extract at 35°C for 15min, repeat 3 times, combine the extracts to obtain an organic extract; use a rotary evaporator to perform rotary evaporation to obtain an organic concentrate, wherein the content of organic solvent is <25%; The concentrated solution was re-dissolved in methanol, wherein the volume ratio of the organic concentrated solution to methanol was 1:1.5, and filtered through a 0.22 μm organic phase filter head to obtain the sample filtrate;

[0036] Install the solid-phase extraction column, activate the solid-phase extraction column with 5mL methanol and 5mL ultrapure water in sequence, a...

Embodiment 2

[0039] A detection method for steroid hormones, other steps are the same as in Example 1, and the difference from Example 1 is: in step (1) sample pretreatment, ultrasonic extraction solvent methanol, ethylene glycol derivatives and ethyl acetate The volume ratio is 1:1:1.

Embodiment 3

[0041] A detection method for steroid hormones, other steps are the same as in Example 1, and the difference from Example 1 is: in step (1) sample pretreatment, a solid-phase extraction column is installed, and activated with 8mL methanol and 8mL ultrapure water successively For the solid phase extraction column, adjust the appropriate vacuum degree, pass 250 μL of the above sample filtrate through the column at a constant flow rate of 3 mL / min, after the sample is loaded, rinse the solid phase extraction column with ultrapure water, and place the extraction column at 40 °C under nitrogen Blow dry, then use 8mL ethyl acetate, 8mL acetonitrile, 8mL ultrapure water eluent to elute the solid phase extraction column successively, the elution rate is 0.5 drops / s, obtain the eluent, and the eluent is constant volume with methanol 2mL, through a 0.22μm microporous membrane, to be tested.

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Abstract

The invention discloses a detection method of steroid hormone and belongs to the technical field of analysis. The method comprises a sample pretreatment step and a liquid mass detection step, wherein the sample pretreatment step comprises ultrasonic extraction and solid-phase extraction; a solvent used for ultrasonic extraction comprises methanol, an ethylene glycol derivative and ethyl acetate; and the ethylene glycol derivative is prepared from ethylene oxide and protozedoary enol. The detection method has high precision and accuracy, and can realize simultaneous extraction and accurate quantification of estrogen, androgen and cortisol hormone in steroid hormone.

Description

technical field [0001] The invention belongs to the technical field of analysis, and in particular relates to a method for detecting steroid hormones. Background technique [0002] Steroid hormones are a class of biologically active compounds that are synthesized and metabolized from cholesterol. According to their biological functions, they can be divided into sex hormones and adrenocortical hormones. Sex hormones mainly include estrogen, androgen, and progesterone, and adrenocortical hormones include glucocorticoids and mineralocorticoids. Many steroid hormone drugs have been widely used in the treatment of humans and animals. Steroid hormones in the environment mainly come from human and animal excreta such as urine and feces and the use and discharge of synthetic steroid hormones. After human and animal excretion, steroid hormones will enter the environment through the outlet of sewage treatment plants or directly discharged by humans. Steroid hormones are a typical ...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/72
CPCG01N30/02G01N30/06G01N30/72G01N2030/027G01N2030/067G01N2030/062Y02P20/52
Inventor 任朝兴
Owner 广西壮族自治区海洋环境监测中心站
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