Vertical micro-fluidic chip and method for PCR detection

Abstract

The invention discloses a vertical micro-fluidic chip and a method for PCR detection. The micro-fluidic chip comprises a chip body, a first communication piston, a second communication piston and a driving piston, wherein the chip body is provided with a separation and purification cavity, a reagent cavity and a reaction cabin; the first communication piston can be rotatably inserted into the chip body and is provided with a first liquid flow groove capable of communicating the separation and purification cavity with the reagent cavity; the second communication piston can be rotatably inserted into the chip body and is provided with a second liquid flow groove capable of communicating the separation and purification cavity with the reaction cabin; the driving piston is movably arranged in the piston cavity of the chip body; the piston cavity is divided into a first piston cavity part and a second piston cavity part by the piston; the first piston cavity part communicates with the separation and purification cavity; the first communication piston is also provided with a first airflow groove capable of communicating the reagent cavity with the second piston cavity part; and the second communication piston is also provided with a second airflow groove capable of communicating the reaction cavity with the second piston cavity part. By use of the vertical micro-fluidic chip, liquid quantification is accurate, and aerosol pollution is avoided.

Description

technical field [0001] The invention belongs to the technical field of PCR detection, and relates to a vertical microfluidic chip and method for PCR detection. Background technique [0002] At present, the microfluidic chip is applied in the field of biological detection, which can be put into a PCR machine for reaction to achieve the purpose of detection, for example, for nucleic acid extraction and amplification. In order to avoid contamination, the reagents required for the reaction are placed in the chamber of the microfluidic chip in advance. During the reaction, the liquid flow direction is controlled according to the set reaction program, and the reagents, samples, and reaction liquids are controlled to flow into the designated chamber. . Therefore, the microfluidic chip is provided with a plurality of pistons used to control the flow direction of the liquid or the communication between the chambers. By rotating or moving these pistons, the communication state of the...

AI Technical Summary

Problems solved by technology

Most of the microfluidic chips currently used for PCR nucleic acid detection are horizontal chips (height is smaller than its size in the horizontal direction), that is, the chamber and the microchannel connecting the chamber are generally located at the same level, and the inside of the chip needs and The outside air is exchanged by air flow, and there are problems of inaccurate liquid quantification and aerosol pollution. Pathogens may enter the outside air and cause pollution.

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