Equipment for conveniently extracting and preparing adipose-derived stem cell exosome and extraction process

Abstract

The invention relates to the technical field of cell preparation, and provides equipment convenient for extracting and preparing adipose-derived stem cell exosomes and an extraction technology. The equipment comprises a separation adjusting frame, an extraction head and a separation frame, the separation adjusting frame comprises a pushing block, and rotating wheels used for adjusting the descending position are movably connected to the two sides of the pushing block; a central rotating rod is movably connected to the side, away from the rotating wheel, of the pushing block, and sliding connecting rods used for adjusting pressure are arranged on the two sides of the central rotating rod correspondingly. According to the equipment for conveniently extracting and preparing adipose-derived stem cell exosome and the extraction process, collected materials are stacked to extrude the air bag frame, so that the air bag frame drives the clamping plate frame and the stabilizing frame through the air column to be tightly buckled and separated into the groove of the rotating block, the rotating stabilizing force in the air bag frame is increased, and the separated precipitate is adipose-derived stem cell exosome, so that the separation stability of the key substance liquid is improved, and the collection efficiency of the adipose-derived stem cell exosome is improved.

Description

technical field [0001] The invention relates to the technical field of cell preparation, in particular to an equipment and an extraction process that facilitates the extraction and preparation of exosomes from adipose stem cells. Background technique [0002] Mesenchymal stem cells are one of the important seed cells, which have the potential of self-renewal and multi-directional differentiation. Their genetic information is stable and can be extracted in large quantities by relatively simple methods. Compared with other sources of mesenchymal stem cells, adipose tissue is a more abundant, consumable and easy-to-obtain tissue. Therefore, adipose-derived mesenchymal stem cells have gradually become a new favorite in stem cell research, and research on adipose-derived mesenchymal stem cells is becoming more and more important. more and more. However, due to the harsh storage conditions and inconvenient transportation of adipose-derived mesenchymal stem cells, clinical applica...

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Problems solved by technology

[0004] However, the current equipment uses centrifugation to collect exosomes. The operation of the extraction equipment is relatively complicated, resulting in a long extraction time, a small amount of extraction, and a low overall extraction efficiency. The unstable centrifugal force makes it difficult to preserve and separate them, and high-speed centrifugation can easily lead to vesicle fragmentation. Therefore, we propose a device and extraction process that facilitates the extraction and preparation of adipose-derived stem cell exosomes.

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