Preparation method of cell chip for non-gynecological tumor screening

Abstract

The invention relates to the technical field of cell chips, and discloses a preparation method of a cell chip for non-gynecological tumor screening. The preparation method comprises the following steps: sampling a cell preserving fluid, placing the cell preserving fluid in a clean centrifuge tube for centrifugation, and placing the centrifuged precipitate in a test tube for preservation; adding the prepared base solution into the test tube, and mixing the base solution with the precipitate to obtain a suspension of the sample cells; taking a glass slide, dividing the glass slide into Sudoku, uniformly dripping the suspension into the Sudoku of the glass slide, and air-drying to obtain the cell chip. According to the preparation method of the cell chip for non-gynecological tumor screening, tumor cells to be screened are dripped on the cell chip when the cell chip is used, then the cell chip is sealed, an image on the cell chip is observed by using a high-power microscope, and the image is extracted and analyzed, so that the tumor cells are screened; nine samples can be screened at one time due to the Sudoku of the cell chip, so that high-efficiency screening is achieved, and the cell chip is prevented from being infected by the sealing structure.

Description

technical field [0001] The invention relates to the technical field of cell chips, in particular to a method for preparing a cell chip for non-gynecological tumor screening. Background technique [0002] Small molecules or gene arrays will be prepared on a chip, and cells applied to it, as the cells connect to each genetic material on the chip, each gene will be slowly released and absorbed into the cell, just like a disease Like vector animals, if the vector animal expresses cell surface receptors, the receptor sequences will be constructed on the cell surface for future assays to screen not only DNA or proteins, but whole cells or organs as well. On a glass surface to make a cell chip or tissue chip, for example, if you want to find a ligand that binds to a cell membrane receptor, you need to arrange the whole cell instead of purifying the receptor and spotting it. If the matrix is ​​composed of Different cell compositions will easily identify cell-specific binding ligand...

AI Technical Summary

Problems solved by technology

[0004] Aiming at the deficiencies of the prior art, the present invention provides a method for preparing a cell chip for non-gynecological tumor screening, which has the advantages of high screening efficiency and a sealed structure to prevent the cell chip from being infected, and solves the problem that is currently prepared. The cell chips are all single chips, the screening efficiency is low, and the cell chip has no sealing structure when used, which leads to the problem of cross-infection between the cell chip and the external environment

Images