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Transport preservation fluid for umbilical cord mesenchymal stem cells and preservation method

A technology of stem cells and preservation methods, which is applied in the field of transport and preservation solution for umbilical cord mesenchymal stem cells, can solve the problems of hidden safety hazards in cell application, complex components, etc., and achieves rapid adaptation to low temperature environment, clear and safe components, and avoidance of increased electrolyte concentration. Effect

Pending Publication Date: 2021-12-21
胡平原
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, low-sugar MDMEM medium and human albumin are required in this preservation solution
Due to the complex composition of the medium, it poses a safety hazard to the subsequent application of cells when used for cell preservation
Human serum albumin is a human blood product. Because the raw material comes from human blood, although the raw plasma has been screened for relevant pathogens, and measures to remove and inactivate viruses have been added to the production process, theoretically there are still transmissions of some known pathogens. The potential risks of known and unknown pathogens should be weighed in clinical use

Method used

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  • Transport preservation fluid for umbilical cord mesenchymal stem cells and preservation method

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Embodiment 1

[0022] A transportation and storage solution for umbilical cord mesenchymal stem cells, which is composed of the following raw materials in parts by weight: 90 parts of sodium chloride injection with a mass concentration of 0.9%, 3.1 parts of α-mangostin, 6 parts of hesperidin, and internal medicine of feverfew 1.8 parts of esters, 5 parts of glucose, 0.7 parts of adenosine, 3 parts of sodium dihydrogen phosphate.

[0023] A method for transporting and preserving umbilical cord mesenchymal stem cells, comprising the following steps: taking 2 mL of preservation solution and precooling to 4° C., adding P2 generation umbilical cord mesenchymal stem cells obtained by subculture, and the cell concentration is 1×10 6 cells / mL and stored at 4°C.

Embodiment 2

[0025] A transport preservation solution for umbilical cord mesenchymal stem cells, which is composed of the following raw materials in parts by weight: 85 parts of sodium chloride injection with a mass concentration of 0.9%, 2.8 parts of α-mangostin, 4.5 parts of hesperidin, and internal 1.6 parts of esters, 3 parts of glucose, 0.5 parts of adenosine, and 1 part of sodium dihydrogen phosphate.

[0026] A method for transporting and preserving umbilical cord mesenchymal stem cells, comprising the following steps: taking 2 mL of preservation solution and precooling it to 6° C., adding P5 generation umbilical cord mesenchymal stem cells obtained by subculture, and the cell concentration is 3×10 6 per mL, stored at 6°C.

Embodiment 3

[0028] A transportation and storage solution for umbilical cord mesenchymal stem cells, which is composed of the following raw materials in parts by weight: 95 parts of sodium chloride injection with a mass concentration of 0.9%, 3.5 parts of α-mangostin, 7.5 parts of hesperidin, and internal medicine of feverfew 2.4 parts of ester, 8 parts of glucose, 1 part of adenosine, 5 parts of sodium dihydrogen phosphate.

[0029] A method for transporting and preserving umbilical cord mesenchymal stem cells, comprising the following steps: taking 2 mL of preservation solution and precooling to 8° C., adding P10 generation umbilical cord mesenchymal stem cells obtained by subculture, and the cell concentration is 5×10 6 per mL, stored at 8°C.

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Abstract

The invention discloses a transport preservation fluid for umbilical cord mesenchymal stem cells. The transport preservation fluid is prepared from the following raw materials: a sodium chloride injection, alpha-mangostin, hesperidin, parthenolide, glucose, adenosine and sodium dihydrogen phosphate. The transport preservation fluid for umbilical cord mesenchymal stem cells takes the sodium chloride injection as a main component, does not contain blood products such as serum, human serum albumin and the like, and has clear and safe components. Three components of alpha-mangostin, hesperidin and parthenolide are also added as protective agents, so that the cell membrane is protected, the situation that the cell internal space structure is disordered due to the fact that the local electrolyte concentration of the cell is increased after the cell is cooled is avoided, the cell is enabled to quickly adapt to a low-temperature environment, and cell inactivation is avoided. The invention further provides a transport preservation method of the umbilical cord mesenchymal stem cells, by adopting the transport preservation fluid, short-term preservation of the cells at 4-8 DEG C is achieved, the survival rate of the cells after being preserved for 3 days is 90% or above, and clinical use requirements are fully met.

Description

technical field [0001] The invention relates to the field of stem cells, in particular to a transport preservation solution and preservation method for umbilical cord mesenchymal stem cells. Background technique [0002] Mesenchymal stem cells are important members of the stem cell family, derived from the mesoderm and ectoderm in the early stages of development. Mesenchymal stem cells were first discovered in the bone marrow, and have attracted increasing attention due to their multiple differentiation potentials, hematopoietic support, promotion of stem cell implantation, immune regulation, and self-replication. Bone marrow mesenchymal stem cells have been widely used clinically, and current research shows that umbilical cord-derived mesenchymal stem cells will also have greater application potential. Umbilical cord mesenchymal stem cells express a variety of unique markers of stem cells. They have the characteristics of high differentiation potential, strong proliferatio...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/0221A01N1/0226
Inventor 胡平原罗耀伟
Owner 胡平原
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