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Bacillus paramushroom preparation for degrading uric acid and preparation method and application of bacillus paramushroom preparation

A technology of Bacillus mycoides and Bacillus, applied in the biological field, can solve the problems of uric acid drug or food with insignificant effect, side effects and the like

Active Publication Date: 2021-12-07
UNIV OF SCI & TECH BEIJING +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The object of the present invention is to provide a biodegradable biodegradable biodegradable biodegradable biodegradable biodegradable biodegradable biodegradable biodegradable biodegradable biodegradable biodegradable biodegradable biodegradable biodegradable biodegradable biodegradable biodegradable biodegradable biodegradable biodegradable biodegradable biodegradable biodegradable biodegradable biodegradable biodegradable biodegradable biodegradable biodegradable biodegradable biodegradable biodegradable biodegradable biodegradable biodegradable biodegradable biodegradable biodegradable biodegradable biodegradable biodegradable biodegradable biodegradable biodegradable biodegradable biodegradable biodegradable biodegradable biodegradable product for the problems of insignificant effects and side effects of drugs or food that degrade and remove uric acid in the prior art [0005] Bacillus of uric acid, the bacteria cells, spores and the enzymes produced by this strain can biodegrade uric acid efficiently, and are safe to human body, and have important application prospects

Method used

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  • Bacillus paramushroom preparation for degrading uric acid and preparation method and application of bacillus paramushroom preparation
  • Bacillus paramushroom preparation for degrading uric acid and preparation method and application of bacillus paramushroom preparation
  • Bacillus paramushroom preparation for degrading uric acid and preparation method and application of bacillus paramushroom preparation

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preparation example Construction

[0072] The third aspect of the present invention provides a method for preparing the Bacillus paramycoides preparation for biodegrading uric acid as described in the second aspect of the present invention, comprising:

[0073] Step B, inoculating the fermentation strain into the fermentation medium. At 18-40°C, preferably 36-38°C, at a shaker speed of 100-300 rpm, after 3-5 days of fermentation culture, a fermentation culture of Bacillus is obtained;

[0074] Step C, performing centrifugation on the fermentation culture of Bacillus, and harvesting the bacteria cells and / or spores of Bacillus;

[0075] Wherein, the fermented strains are obtained from corresponding bacterial strains through seed cultivation.

[0076] As known to those skilled in the art, at present, 16S rRNA is often used internationally for molecular identification of bacteria, so 16S rRNA can be used for comparison of similarities to obtain their homology. Therefore, the fermentation strains used in the pres...

Embodiment approach

[0092] According to some embodiments of the present invention, the preparation method also includes:

[0093] Step K, performing cell disruption treatment on the cell suspension of Bacillus in an ice-water bath (i.e. ice-water mixture, 0-4° C.) to obtain a cell-free disruption solution of Bacillus;

[0094] In step L, the cell-free lysate of Bacillus is centrifuged, and the supernatant cell-free extract is taken as the crude enzyme of Bacillus paramycoides YC-02 strain.

[0095] The present invention has no particular limitation on the conditions of the centrifugation in the above step L. In some embodiments of the present invention, for example, the centrifugation of the to-be-separated product can be performed at 15000-18000 r / min for 10-20 min.

[0096] The fourth aspect of the present invention provides a preparation of Bacillus paramycoides that biodegrades uric acid according to the second aspect of the present invention or the Bacillus paramycoides that biodegrades uric...

Embodiment 1

[0113] (1) Prepare a growth medium for Bacillus paramycoides YC-02, which consists of (per liter): 10.0 g of whole milk powder, 10.0 g of yeast powder, and 5.0 g of NaCl. Add 100 milliliters of the prepared liquid culture medium into a 500 milliliter triangular flask, sterilize at high temperature and pressure (121° C.) for 20 minutes, and then sterilize for another 20 minutes under ultraviolet irradiation in a clean workbench.

[0114] (2) Under sterile conditions in a clean bench, inoculate 0.5 ml of Bacillus paramycoides YC-02 bacterial solution into 100 ml of liquid medium in a conical flask, at a temperature of 38°C and a shaker speed of 200 rpm. After performing batch culture for 3 days, the supernatant was poured off after centrifugation (8000 rpm, 10 minutes), and the Bacillus paramycoides YC-02 cells and / or spores were harvested.

[0115] Take 20mL of Bacillus paramycoides YC-02 cell suspension, add it to a 50mL glass tube, insert it into ice water, and use an ultraso...

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Abstract

The invention belongs to the technical field of biology, and relates to bacillus for biodegradation of uric acid. The bacillus can generate enzyme for catalytic degradation of the uric acid, and the bacillus is a bacillus parabarisiformis YC-02 strain. The invention further relates to a bacillus paramushroom preparation for biodegrading uric acid. The bacillus paramushroom preparation contains one or more of bacterial cells, spores and crude enzyme of the bacillus. Research results show that the bacillus for biodegrading uric acid and the enzyme produced by the bacillus are safe to human bodies, can efficiently biodegrade uric acid, and have important application prospects in the aspects of removing uric acid and treating human body hyperuricemia and corresponding complications.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a preparation of Bacillus paramycoides for degrading uric acid, a preparation method and application thereof. Background technique [0002] Since the beginning of the 21st century, my country's social and economic level has been continuously improved, and the diet has become increasingly diversified. With the increase in the intake of foods with high fat and purine content, the incidence of many diseases has also continued to increase. Hyperuricemia (HUA) will is one of them. Relevant epidemiological studies have shown that the global prevalence of HUA is 5% to 25%, and it is another common metabolic disease after diabetes. According to studies and reports on the prevalence of HUA in various parts of my country in recent years, the number of HUA patients in mainland China is estimated to have reached 200 million. Middle-aged and elderly men and postmenopausal women are the high-risk g...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/742A61P19/06C12R1/07
CPCC12N1/20A61K35/742A61K9/0053A61P19/06
Inventor 闫海蔡静远邹倩文姜尧杨瑞民刘晓璐张海洋吴安情徐止开
Owner UNIV OF SCI & TECH BEIJING
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