Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for culturing upper urinary tract urothelial cell carcinoma organoids

A culture method and epithelial technology, applied in the field of organoid culture

Inactive Publication Date: 2021-12-03
深圳明澳生物科技有限公司
View PDF0 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, since UTUC is a relatively rare tumor of the genitourinary system, the use of chemotherapy drugs in clinical practice is often based on inherited empirical prescriptions or personal treatment experience of doctors, and cannot be used for the use of chemotherapy drugs and the development of upper urinary tract urothelial tumors. Personalized Therapy Delivers Reliable Clinical Data

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for culturing upper urinary tract urothelial cell carcinoma organoids
  • Method for culturing upper urinary tract urothelial cell carcinoma organoids
  • Method for culturing upper urinary tract urothelial cell carcinoma organoids

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0047] The following will clearly and completely describe the technical solutions in the embodiments of the application with reference to the drawings in the embodiments of the application. Apparently, the described embodiments are only some of the embodiments of the application, not all of them. Based on the embodiments in this application, all other embodiments obtained by those skilled in the art without making creative efforts belong to the scope of protection of this application.

[0048] The terms "first" and "second" in this application are used to distinguish different objects, not to describe a specific order. Furthermore, the terms "include" and "have", as well as any variations thereof, are intended to cover a non-exclusive inclusion. For example, a process, method, system, product, or device that includes a series of steps or modules is not limited to the listed steps or modules, but optionally also includes steps or modules that are not listed, or optionally inclu...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for culturing upper urinary tract urothelial cell carcinoma organoids. The method comprises the following steps: placing small upper urinary tract urothelial cell carcinoma tissue blocks in an inhibitor containing type II collagenase for first digestion treatment to obtain a first digest; placing the first digest in a pancreatin substitute containing an inhibitor, and performing second digestion treatment to obtain a second digest; sequentially performing first neutralization treatment, filtering treatment and centrifugal treatment on the second digest to obtain single cell precipitate; placing the single cell precipitate in a mixed substrate of a preset culture medium and substrate glue for resuspension treatment to obtain a gel suspension containing all cells; and culturing the gel suspension according to a preset scheme to obtain the upper urinary tract urothelial cell carcinoma organoids. According to the scheme, reliable clinical data can be provided for use of chemotherapeutic drugs and personalized treatment of upper urinary tract urothelial cell tumors.

Description

technical field [0001] The present application relates to the technical field of organoid culture, in particular to a method for culturing organoids of upper urinary tract urothelial carcinoma. Background technique [0002] Upper Urinary Tract Urothelial Cell Carcinoma (UTUC) is a rare tumor of the genitourinary system that originates from the urothelial lining of the upper urinary tract. UTUC accounts for 5-10% of all urothelial carcinomas and 10% of renal cancers. Despite the low incidence of UTUC, it is characterized by high mortality and recurrence rates, 60% of cases are aggressive at the time of diagnosis, the 5-year survival rate of patients with pT2 / pT3 invasion of the muscular wall is less than 50%, and pT4 The 5-year survival rate of patients is less than 10%. [0003] For patients with advanced upper tract urothelial carcinoma, radical nephroureterectomy combined with bladder cuff resection is still the standard treatment for UTUC, systemic chemotherapy and neoa...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/09C12N5/071
CPCC12N5/0693C12N5/0684C12N2513/00
Inventor 余磊陈洁琳吴万军余建辉
Owner 深圳明澳生物科技有限公司
Features
  • Generate Ideas
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com