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Probiotic strain for improving intestinal barrier function and application thereof

A technology of probiotics and bacterial strains, applied in the field of probiotics, can solve the problem of not many bacterial strains, achieve the effects of relieving colitis and systemic inflammation symptoms, prolonging life, and wide application prospects

Active Publication Date: 2021-12-03
SHANGHAI FIRST PEOPLES HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at the problem that there are not many bacterial strains for improving intestinal barrier function, the present invention provides a group of probiotic strains for improving intestinal barrier function, and discloses the group Application of probiotic strains in the preparation of products that improve intestinal barrier function impairment and related developmental disease symptoms and delay aging

Method used

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  • Probiotic strain for improving intestinal barrier function and application thereof
  • Probiotic strain for improving intestinal barrier function and application thereof
  • Probiotic strain for improving intestinal barrier function and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Example 1 The protective effect of bacterial strains of the present invention on intestinal barrier function

[0051] 1. Experimental method

[0052] Establishment of intestinal barrier injury mouse model and drug treatment: C57BL / 6 mice aged 6 to 8 weeks were randomly divided into 6 groups (5-7 mice in each group) after 1 week of adaptation, and they were normal controls group (PBS group), model control group (PBS+DSS group) and four strain intervention groups (DSS+LP group, DSS+PA group, DSS+EF group, DSS+EC group), PBS group was given drinking pure water, And 200 μl of PBS was administered by stomach every day; the other 5 groups were given to drink 4% DSS water for 5-6 days, and the PBS+DSS group was given 200 μl of PBS every day while drinking DSS water; the other 4 groups were given DSS water At the same time as water, 200μl containing 10 9 A bacterial suspension of Lactobacillus plantarum LP, Pediococcus lactis PA, Enterococcus faecium EF or Escherichia coli EC...

Embodiment 2

[0060] Example 2 The alleviating effect of bacterial strains of the present invention on colonic inflammation

[0061] 1. Experimental method

[0062] (1) In vitro experiment

[0063] Establishment and treatment of HT-29 colon cell model: Add tumor necrosis factor α (TNF-α) and 10 7 A single bacterial strain of the present invention was established, and a control group (Control group) without any bacterial strain was established at the same time, and co-cultured for 4 hours.

[0064] After the culture, the cell supernatant was collected, protease inhibitors were added, and the concentrations of colonic inflammatory factors IL-1β and IL-6 were detected by ELISA.

[0065] (2) In vivo experiment

[0066] Establishment and treatment of DSS colitis mouse model: C57BL / 6 mice aged 6 to 8 weeks were randomly divided into 6 groups (5-7 mice in each group) after 1 week of adaptation, and they were normal control group ( PBS group), model control group (PBS+DSS group) and 4 strain in...

Embodiment 3

[0077] Embodiment 3 The improvement effect of bacterial strains of the present invention on systemic inflammation

[0078] 1. Experimental method

[0079] The animal modeling process is the same as the establishment and treatment process of the DSS colitis mouse model in Example 2. After the treatment, the body weight is measured and the data is recorded; the mice are sacrificed, the colon is collected and total RNA is extracted, and LPS is detected by real-time quantitative PCR The mRNA expression level of the receptor TLR4; in addition, blood was collected, serum was prepared, and the content of C-reactive protein in serum was detected by ELISA method.

[0080] 2. Experimental results

[0081] Experimental results such as image 3 As shown, compared with the normal control group, the body weight of the model control group mice significantly decreased, and the mRNA expression level of LPS receptor TLR4 in the colon tissue and the content of C-reactive protein in the serum a...

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Abstract

The invention discloses a probiotic strain for improving intestinal barrier function and application thereof, and relates to the field of probiotics. The probiotic strains provided by the invention are lactobacillus plantarum, pediococcus acidilactici, enterococcus faecium and escherichia coli; wherein the microbial preservation number of the lactobacillus plantarum is CGMCC No.17941; the microbial preservation number of the pediococcus acidilactici is CGMCC No.17943; the microbial preservation number of the enterococcus faecium is CGMCC No.17944; and the microbial preservation number of the escherichia coli is CGMCC No.17945. The probiotic strain provided by the invention can significantly inhibit the increase of intestinal permeability caused by intestinal barrier function damage and recover the intestinal permeability to a healthy level; can effectively relieve colitis and systemic inflammation symptoms caused by intestinal barrier function damage; in addition, the probiotic strain also has the effects of delaying senescence and prolonging the service life. The probiotic strain can be widely applied to products such as drugs for improving intestinal barrier function damage and related development diseases and delaying senescence, and the market application value is high.

Description

technical field [0001] The invention belongs to the field of probiotics, and relates to probiotic bacterial strains for improving intestinal barrier function and applications thereof. Background technique [0002] The intestinal barrier is the first line of host defense, including physical barriers (mucin secreted by goblet cells; epithelial tight junctions, adherens junctions, etc.) and immune barriers (cytokines, chemokines secreted by immune cells and epithelial cells, etc. ). A healthy and complete intestinal barrier can prevent microorganisms and endotoxins (LPS) from entering the body's circulation, maintain normal intestinal function, and ensure the health of the body. Once the intestinal barrier function is damaged, the intestinal permeability increases, and the endotoxin in the intestinal lumen breaks through the intestinal barrier and enters the circulation of the body, which promotes the release of a large number of inflammatory factors, causes inflammation in th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/747A61K35/744A61K35/741A61P1/00A61P1/04A61P29/00A61P39/06A23L33/135C12R1/25C12R1/19C12R1/01
CPCA61K35/744A61P1/00A61P29/00A61P39/06A23L33/135A23V2002/00C12N1/205C12R2001/46A23V2200/32A23V2200/3204A23V2200/302Y02A50/30
Inventor 王兴鹏王京晶代娟娟王晓鑫郎涛江明杰
Owner SHANGHAI FIRST PEOPLES HOSPITAL
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