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Method for enhancing stability of early warning against water environment toxicity

A technology of stability and water environment, which is applied in the direction of measuring devices, instruments, and material analysis through electromagnetic means, can solve the problem of high cost, achieve the effects of reducing impact, facilitating rapid recovery, and avoiding the impact of fluctuations

Pending Publication Date: 2021-11-26
CHONGQING INST OF GREEN & INTELLIGENT TECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The results show that after adding the inducer, even if the MFC sensor contains 10mg / L Cu 2+ Water samples are tested, and the voltage can also be restored to the original state, but this method has the problem of high cost, and further research is needed to improve the recovery ability of anode microorganisms

Method used

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  • Method for enhancing stability of early warning against water environment toxicity
  • Method for enhancing stability of early warning against water environment toxicity
  • Method for enhancing stability of early warning against water environment toxicity

Examples

Experimental program
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Effect test

Embodiment 1

[0036] A new differential coulometric method for early warning of toxicity in the water environment, the process is as follows figure 1 As shown, first add sodium acetate reagent to the water sample to be tested to achieve the purpose of sufficient BOM in the sample. On the one hand, sodium acetate is selected as the additive agent because sodium acetate is easily degraded and metabolized by the anode biofilm, so that the enzyme utilization rate of microorganisms on the anode can quickly reach saturation; on the other hand, compared with other types of BOM, sodium acetate is not easily compatible Toxic substances produce complex precipitation, which can ensure the accuracy of early warning of water toxicity.

[0037] 1. According to the Mie equation (formula 1.1), when the substrate concentration exceeds a certain concentration, the utilization rate of enzymes in microorganisms can reach 100%, and the concentration of organic matter is no longer a factor affecting its degradat...

Embodiment 2

[0047] The differential coulometric method is used to carry out the early warning of the toxicity of the water environment. The specific method is as follows:

[0048] (1) Prepare the MFC sensor: use carbon cloth (wherein the carbon cloth has been cultivated in a large battery cultured in a long-term sodium acetate matrix for more than one month) as the anode material, and the air cathode carbon cloth material (including the carbon cloth of the base layer and the carbon cloth coated separately) A waterproof layer and a catalytic layer brushed on the base layer, the waterproof layer is a mixture of a plasticizer and an adhesive, and the catalytic layer Pt / C, wherein the mass ratio of Pt and C is 2:8) is a cathode material ;

[0049] (2) Sodium acetate is added to the water sample to be tested until the concentration of sodium acetate in the water sample is 384.6mg / L, and the corresponding standard BOD 5 Values ​​were 200mg / L, and then the MFC sensor was placed in the water sam...

Embodiment 3

[0053] 1. Selection of acquisition signal

[0054] Taking carbon cloth (wherein the carbon cloth is cultivated in a large battery cultivated in a long-term operation of sodium acetate matrix for more than one month) as the anode material, air cathode carbon cloth material (comprising base layer carbon cloth and brushing on the base layer respectively) Waterproof layer and catalytic layer, described waterproof layer is the mixture of plasticizer and binding agent, described catalytic layer Pt / C, wherein the mass ratio of Pt and C is 2: 8) is cathode material; In the water sample to be tested Add sodium acetate until the concentration of sodium acetate in the water sample is 192.3mg / L, 384.6mg / L, 576.9mg / L, 961.5mg / L and 1538.4mg / L respectively, the corresponding standard BOD 5 The values ​​are 100mg / L, 200mg / L, 300mg / L, 500 and 800mg / L respectively, and the voltage acquisition curves of its MFC sensor degrading different concentrations of sodium acetate are as follows Figure ...

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Abstract

The invention relates to a method for enhancing the stability of early warning against water environment toxicity, and belongs to the technical field of water body biotoxicity early warning. According to the method, a differential coulometric method is adopted, and the principle of the differential coulometric method is as follows: adding sodium acetate with the concentration of more than or equal to 384.6 mg / L into a water sample to be detected, enabling the utilization rate of microbial enzyme on an anode to be saturated, collecting coulometric quantity after the sodium acetate is added for 2-5 hours as an analytic signal, and calculating the inhibition ratio (IR) of the coulometric quantity to perform early warning of toxicity.

Description

technical field [0001] The invention belongs to the technical field of water body biological toxicity early warning, and relates to a method for enhancing the stability of water environment toxicity early warning. Background technique [0002] In recent years, MFC (microbial fuel cell) sensors have been widely used in the detection of various water quality indicators, especially in the early warning of water environmental toxicity with strong practicability. Toxic substances can inhibit the electrochemical activity of the anode biofilm, resulting in the weakening of the electrical signal of the MFC sensor. The toxicity pollution of the water environment can be judged according to the inhibition rate (Inhibition Ration, IR) of the electrical signal. From the existing research, it is known that BOM (biodegradable organic matter) can give nutrients to the anode microorganisms and participate in the generation of MFC sensor electrons. When the MFC sensor is used for toxicity wa...

Claims

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Application Information

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IPC IPC(8): G01N27/42
CPCG01N27/423
Inventor 刘鸿高阳阳宋诚王厦刘元
Owner CHONGQING INST OF GREEN & INTELLIGENT TECH CHINESE ACADEMY OF SCI
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