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Kit for early diagnosis of gallbladder cancer and application thereof

An early diagnosis and kit technology, applied in the field of biomedicine, can solve the problems of less molecular research on pathogenesis and carcinogenesis, and poor overall survival

Pending Publication Date: 2021-11-26
普瑞基准科技(北京)有限公司 +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] GBC is a malignancy with poor overall survival and relatively little molecular research on its pathogenesis and carcinogenesis

Method used

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  • Kit for early diagnosis of gallbladder cancer and application thereof
  • Kit for early diagnosis of gallbladder cancer and application thereof
  • Kit for early diagnosis of gallbladder cancer and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] experimental method:

[0029] Patient Recruitment and Sample Cohorts

[0030] All samples were collected under the approval of the Institutional Review Board (IRB) of Peking Union Medical College Hospital (PUMCH), China. Informed and written consent was obtained from each patient. The present invention recruited 11 patients who underwent surgical resection of gallbladder polyps at PUMCH from April 2008 to December 2016, and pathologically confirmed the coexistence of GBC, GBA and GIN lesions physically. GBC, GBA, GIN, and normal gallbladder tissue are required for each patient sample. To minimize carcinogenic effects on normal samples, the present invention included only normal gallbladder tissue in the cancer-free paraffin blocks. All clinical data were obtained from hospital records.

[0031] Whole Exome Sequencing

[0032] Serial 5-μm thick serial sections were cut from representative dewaxed stone blocks and stained with hematoxylin. Multiple pathologists inde...

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PUM

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Abstract

The invention relates to a kit for early diagnosis of gallbladder cancer and application thereof. The kit comprises a CTNNB1 mutation detection reagent and an ARID2 mutation detection reagent. According to the invention, senescence is determined to be a main factor for promoting accumulated mutations and CTNNB1 and ARID2 mutations in the tumors, especially CTNNB1 mutation plays a key role. According to the invention, a foundation is laid for early diagnosis and effective treatment of gallbladder cancer through the understanding of reconstruction of gallbladder cancer transformation and canceration evolution trajectory.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a gallbladder cancer early diagnosis kit and application thereof. Background technique [0002] Gallbladder cancer (GBC) is the most common biliary tract cancer. Its incidence shows large geographical variations, with rates approaching 30 per 100,000 women in some countries, such as Chile and India. Unlike other types of cancer, only 30% of GBC patients are diagnosed or suspected preoperatively, while other cases are diagnosed based on postoperative incidental findings. In most patients, tumors were identified at the time of surgery or when surgical specimens were examined by a pathologist. Most patients with symptomatic GBC have incurable tumors, and even after R0 resection, the risk of GBC recurrence is 64% at 5 years. The clinical outcome of GBC is very poor, with an overall 5-year survival rate of less than 5%, although a 75% 5-year survival rate can be ach...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886
Inventor 赵海涛林振健彭鑫鑫季序我
Owner 普瑞基准科技(北京)有限公司
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