Cryopreservation solution and cryopreservation method for immune cells

Abstract

The invention relates to a cryopreservation solution and a cryopreservation method for immune cells. The cryopreservation solution comprises FBS, DMSO, a 1640 culture solution, low-molecular heparin calcium and an antioxidant. The cryopreservation method of the cryopreservation solution for immune cells comprises the following steps: 1, storing: putting the immune cells into a freezing tube, then adding a freezing medium, slowly blowing and beating, uniformly mixing, and putting the freezing tube into a cooling chamber; 2, cooling for the first time: cooling to 3 DEG C and keeping the temperature for 4 minutes; then cooling to -3 DEG C at a speed of -2 DEG C / min; and finally, reducing the temperature to -40 DEG C at the speed of -11 DEG C / min; 3, raising the temperature to -30 DEG C at the speed of -16 DEG C / min for the first time; 4, cooling for the second time: keeping the temperature at -30 DEG C for 4 minutes; then cooling to -50 DEG C at the speed of -2 DEG C / min; finally, reducing the temperature to -80 DEG C at the speed of -10 DEG C / min; and keeping at -80 DEG C for 5 days; and 5, storing: finally storing in a liquid nitrogen tank for cryopreservation.

Description

technical field [0001] The invention relates to the production of moxa moxibustion strips of Miao medicine, in particular to a freezing solution and freezing method of immune cells. Background technique [0002] Cell cryopreservation is a technology that places cells in a low temperature environment to slow down cell metabolism to achieve long-term storage. The principle is to store the cells at -196°C in liquid nitrogen at ultra-low temperature, which can make the cells temporarily out of the growth state and preserve the cell characteristics, and can be used by resuscitating the cells when needed. Adding a protective agent to the cell suspension during cryopreservation can lower the freezing point of the solution. Under the condition of slow freezing, the water in the cells penetrates out of the cells, reducing the formation of ice crystals in the cells, thereby avoiding damage to the cells during freezing. At present, cell cryopreservation is mainly stored under the ultr...

AI Technical Summary

Problems solved by technology

However, in the existing cryopreservation methods of immune cells, only the cryopreservation of stem cells and tumor cells has been solved, while the method of cryopreservation of immune cells has not been well solved.

Due to the cell structure and cell characteristics of immune cells, when using the previous cell freezing solution and cryopreservation method to freeze immune cells, the immune cells are easily damaged by ice crystals, the cell viability is low after recovery, the number of cell proliferation is insufficient, and the cells are prone to aging. Low efficiency, low quality of cryopreservation, poor activity of cells after recovery, and low survival rate

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