Single-base extension primer group and method for detecting polymorphism of vitamin A metabolism related gene
A single-base extension, gene polymorphism technology, applied in biochemical equipment and methods, microbial determination/inspection, recombinant DNA technology, etc., can solve the problems of low detection efficiency, long detection cycle, and low specificity, etc. Achieve the effect of high detection efficiency, accurate results and high extension efficiency
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[0105] A method for detecting gene polymorphisms related to vitamin A metabolism, comprising the following steps:
[0106] S1. Design multiple PCR amplification primer sets and single-base extension primer sets respectively according to the polymorphic sites of genes related to vitamin A metabolism; extract the genomic DNA of the sample to be tested.
[0107] (1) Multiplex PCR amplification primer set and single base extension primer set design and results:
[0108] When designing multiplex PCR amplification primer sets and single-base extension primer sets, 15 polymorphic sites of 6 genes related to vitamin A metabolism were selected according to the results of literature research, as shown in Table 1 for details. Then search the DNA sequence of each site through NCBI, design and synthesize the best multiplex PCR amplification primer set and single base extension primer set, the design principle is: to ensure that the amplified fragments will not interact to produce non-speci...
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