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Combination medicine for treating cancer

A cancer and drug technology, applied in the field of medicine, can solve problems such as narrow indications and easy drug resistance, and achieve strong anti-tumor effects and good synergistic effects

Inactive Publication Date: 2021-10-08
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, many PARP inhibitors, including olaparib, have limitations of narrow indications and prone to drug resistance

Method used

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  • Combination medicine for treating cancer
  • Combination medicine for treating cancer
  • Combination medicine for treating cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1 PARP1 inhibitors combined with FOXM1 inhibitors inhibit the growth of triple-negative breast cancer and pancreatic cancer cells in vitro:

[0047] 1. Experimental method:

[0048] (1), MTT detection of cell proliferation:

[0049] Two kinds of tumor cells were treated with DMSO (control), olaparib, FDI-6, and olaparib and FDI-6 in combination, wherein the molar ratio of olaparib and FDI-6 in the combined treatment group was 1:( 0.5~2); After 7 days, the cell proliferation was detected by MTT;

[0050] (2), colony formation assay to detect the number of cells:

[0051] The effect of combination drugs on cell proliferation was detected by clone formation assay, two kinds of tumor cells were treated with olaparib combined with FDI-6 (MDA-MB-231 olaparib 2.0μM, FDI-6 2.0μM; SW1990 olaparib 3.0 μM, FDI-6 3.0 μM); 10 days later, fix with 4% paraformaldehyde for 15 minutes, stain with crystal violet for 10 minutes, observe and take pictures with an inverted fluore...

Embodiment 2

[0062] Example 2 Olaparib combined with FDI-6 inhibits triple-negative breast cancer xenograft tumor growth in vivo

[0063] (1), the test drug:

[0064] PARP inhibitor: olaparib; FOXM1 inhibitor: FDI-6;

[0065] Preparation method: Olaparib is prepared with 10% DMSO and 10% β-cyclodextrin; FDI-6 is prepared with 10%

[0066] Prepared with DMSO, 10% Tween and 10% β-cyclodextrin;

[0067] (2), experimental animals:

[0068] BALB / cA-nude nude mice, 6-8 weeks old, female; feeding environment: SPF grade;

[0069] (3) Experimental steps:

[0070] Nude mice were subcutaneously inoculated with human triple-negative breast cancer MDA-MB-231 cells, and the tumors grew to 100-300mm 3 Afterwards, the animals were randomly divided into control group, olaparib group, FDI-6 group and combined administration group; the olaparib group was injected intraperitoneally with olaparib (60mg / kg), once a day; Group through intraperitoneal injection of FDI-6 (60mg / kg), once a day; combination gr...

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Abstract

The invention provides a combination medicine for treating cancer, and belongs to the technical field of medicines. The combination medicine comprises a PARP1 inhibitor and an FOXM1 inhibitor. The two inhibitors have the limitations of narrow indications, easiness in drug resistance and the like when independently used for treating tumors. The medicine composition provided by the invention can obtain a very strong anti-tumor effect according to an effective administration dosage and an administration mode, and shows a relatively good synergistic effect. Particularly, the medicine composition provided by the invention shows a stronger effect than a single medicine in inhibiting the growth of tumors such as triple-negative breast cancer and pancreatic cancer, and provides a new way for tumor treatment.

Description

technical field [0001] The invention belongs to the technical field of medicine, and relates to a combined drug for treating cancer, in particular, to a pharmaceutical composition with PARP1 inhibitor and FOXM1 inhibitor as active ingredients, a preparation method thereof and its use for treating cancer. Background technique [0002] With the change of people's lifestyle, the incidence of tumor is getting higher and higher, which seriously threatens the life and health of human beings. At present, the incidence of breast cancer ranks first, and it is one of the most common cancers threatening women all over the world. Triple-negative breast cancer is a highly heterogeneous tumor, accounting for 15%-20% of breast cancer pathological subtypes. Due to the lack of expression of estrogen receptor, progesterone receptor, and human epidermal growth factor receptor 2, triple-negative breast cancer cannot be targeted by endocrine and other methods, and it is a subtype of breast canc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K45/06A61K31/502A61K31/4365A61P35/00
CPCA61K45/06A61K31/502A61K31/4365A61P35/00A61K2300/00
Inventor 徐云根王淑平吴诗琪林倩文黄诗卉唐艺轩孟柳琼
Owner CHINA PHARM UNIV
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