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Serum potassium detection reagent freeze-dried microsphere and preparation method thereof

A technology for detecting reagents and serum potassium, applied in the field of serum potassium detection reagent freeze-dried microspheres and preparation thereof, can solve the problems of difficult carrying, complicated operation and high cost

Pending Publication Date: 2021-10-01
重庆东渝中能实业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The present invention intends to provide a serum potassium detection reagent freeze-dried microsphere with good stability, strong specificity and easy operation and its preparation method, so as to solve the problems of complicated operation, high cost and difficult carrying in the detection method in the prior art. The problem

Method used

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  • Serum potassium detection reagent freeze-dried microsphere and preparation method thereof
  • Serum potassium detection reagent freeze-dried microsphere and preparation method thereof
  • Serum potassium detection reagent freeze-dried microsphere and preparation method thereof

Examples

Experimental program
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Embodiment 1

[0030] A preparation method for serum potassium detection reagent freeze-dried microspheres, comprising the steps of:

[0031] S1: preparation of the serum potassium detection reagent, the composition of the serum potassium detection reagent in this embodiment is as follows:

[0032] Table 1

[0033] components specific components concentration buffer Tris-cl (pH 8.4) 200mmol / L GDH GDH 12KU / L PK PK 3KU / L α-KG α-KG 3mmol / L LDH LDH 26KU / L ADP ADP 6mmol / L PEPs PEPs 6mmol / L NADH NADH 0.35mmol / L Acupoint Mixture K221 5mmol / L Substrate protectant PEG20000 1％ Substrate protectant PEG8000 0.5％ Substrate protectant β-cyclodextrin 0.5％ Surfactant Triton X-100 0.1％ freeze-dried stabilizer Trehalose 0.05％ preservative Proclin-300 0.01％

[0034] The serum potassium detection reagent is obtained by mixing the above-mentioned raw materials uniforml...

Embodiment 2

[0045] The difference between this example and Example 1 is: in this example, S1: preparation of serum potassium detection reagent, the composition of serum potassium detection reagent in this embodiment is as follows:

[0046] table 3

[0047]

[0048]

[0049] The serum potassium detection reagent is obtained by mixing the above-mentioned raw materials uniformly in proportion.

[0050] S2: Preparation of freeze-dried microspheres. The prepared serum potassium detection reagent is separated into droplets (10ul / drop) in liquid nitrogen by an automatic liquid separation system, and the detection reagent forms microspheres in liquid nitrogen, and then freeze-dried The freeze-drying process is carried out in the machine. In this embodiment, the freeze-drying includes a pre-freezing stage, a sublimation drying stage and an analysis drying stage. The conditions of the pre-freezing stage are: -50°C, 60min; the conditions of the sublimation drying stage are: -30°C, 60min; -20°...

Embodiment 3

[0060] The difference between this example and Example 2 is that in this example, the reagent K221 is replaced with 15-crown-5. For the preparation of freeze-dried microspheres, the prepared serum potassium detection reagent was separated into droplets (10ul / drop) in liquid nitrogen using an automatic liquid separation system, and the detection reagent was formed into microspheres in liquid nitrogen, and then placed in a freeze dryer. Freeze-drying was carried out. The detection procedure is the same as the grouping and detection method of Example 2.

[0061] The composition of serum potassium detection reagent in the present embodiment is as follows:

[0062] table 5

[0063] components specific components concentration buffer Tris-cl (pH 8.4) 200mmol / L GDH GDH 12KU / L PK PK 3KU / L α-KG α-KG 3mmol / L LDH LDH 26KU / L ADP ADP 6mmol / L PEPs PEPs 6mmol / L NADH NADH 0.35mmol / L Acupoint Mixture ...

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Abstract

The invention relates to the field of detection reagent preparation, and discloses serum potassium detection reagent freeze-dried microspheres and a preparation method thereof, the freeze-dried microspheres are formed by freeze-drying a detection reagent, and the detection reagent is prepared from 50-500 mmol / L of a buffer solution, 10-100 KU / L of GDH, 2-20 KU / L of PK, 2-10 mmol / L of alpha-KG, 20-200 KU / L of LDH, 5-50 mmol / L of ADP, 5-50 mmol / L of PEP, 0.3-2 mmol / L of NADH, 5-50 mmol / L of a hole mixture, 0.5%-2% of a substrate protective agent, 0.1%-1% of a surfactant, 0.05%-2% of a freeze-drying stabilizer and 0.01%-0.1% of a preservative. After the detection reagent is prepared, liquid separation and bead dropping are carried out to form microspheres, and then freeze drying is carried out to form freeze-dried microspheres. During detection, a biochemical analyzer is adopted, a two-point rate method is used for detection, the detected main wavelength is 340 nm, and the detected auxiliary wavelength is 410 nm. The stability and the anti-interference capability of the reagent are improved, the reagent is easy to store and carry, one-time detection and use are convenient, and the detection efficiency is improved.

Description

technical field [0001] The invention relates to the field of detection reagent preparation, in particular to a serum potassium detection reagent freeze-dried microsphere and a preparation method thereof. Background technique [0002] Potassium (K) is one of the important electrolytes to maintain cell metabolism, regulate body fluid osmotic pressure, maintain acid-base balance and maintain cell stress function. About 98% of potassium in the human body exists in cells, and the remaining 2% exists in blood and body fluids. Potassium ions in the human body depend entirely on external intake, and the daily intake of potassium from food is about 50-100 mmol. Serum potassium refers to the concentration of potassium ions in the serum. The normal serum potassium concentration is 3.5-5.5mmol / L. When the serum potassium is <3.5mmol / L, it is called hypokalemia, and when the serum potassium is >5.5mmol / L, it is called hyperkalemia. Potassium. [0003] At present, the main method...

Claims

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Application Information

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IPC IPC(8): G01N21/31G01N21/33G01N1/38
CPCG01N21/314G01N21/33G01N1/38
Inventor 胡军瑜陈枫李诗雨
Owner 重庆东渝中能实业有限公司
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