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Primer pair for microalgae identification and application thereof

A primer pair and microalgae technology, applied in the fields of molecular biology and bioinformatics, can solve problems such as the inability to identify "family, genus and species", poor universality of primers, and lag in DNA barcode research

Active Publication Date: 2021-10-01
INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

[0004] In general, the research on DNA barcoding of microalgae is still very lagging behind, and the existing primers have poor versatility and low resolution
Currently developed DNA barcodes, such as ITS and 18S rDNA, can only identify "phylum" or "order", but cannot identify "family, genus and species"

Method used

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  • Primer pair for microalgae identification and application thereof
  • Primer pair for microalgae identification and application thereof
  • Primer pair for microalgae identification and application thereof

Examples

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Embodiment Construction

[0021] The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention. Unless otherwise specified, the examples were carried out according to conventional implementation conditions. The test materials used in the following examples, unless otherwise specified, were purchased from conventional biochemical reagent manufacturers.

[0022] 1. The nucleotide sequence of the primer pair

[0023] By comparing the whole chloroplast genome sequences of Chaetoceros mourii, Chaetoceros teniosa and Chaetoceros simplex, a pair of universal primers was developed by using the conserved flanking sequences of hypervariable regions:

[0024] Primer pair name: PsaA-PsaB

[0025]Primer 1 (cpDNAB2F): 5'-TCTTATGGTCACAAGCTTCTCA-3', and

[0026] Primer 2 (cpDNAB2R): 5'-CTGTCATACCATCATGTGCTTC-3'.

[0027] 2. Extract the total DNA of each microalgae

[0028] The 21 kinds of microalgae studied in the present invention have semin...

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Abstract

The invention provides a primer pair for microalgae identification, wherein the primer pair is cpDNAB2F: 5'-TCTTATGGTCACAAGCTTTCCA-3', and cpDNAB2R: 5'-CTGTCATACCATATGTGCTTCC-3'. According to the present invention, the 21 microalgae are subjected to PCR amplification by using the primer pair so as to respectively obtain the unique chloroplast DNA bar code sequences, wherein the 11 microalgae such as Seminavis robus, Nitzschia palea, Chaetoceros muelleri, Picocystis salina and the like can be identified into the species, and the other microalgae species can be identified into the genus, such that the repeatability is good, the stability and the reliability are good, and the detection is rapid.

Description

technical field [0001] The invention relates to molecular biology and bioinformatics, in particular to a pair of primers for identification of microalgae and its application. Background technique [0002] Marine microalgae is a general term for a group of microscopic (2-30 micron) single-celled organisms capable of photosynthesis, including eukaryotic and prokaryotic, mainly cyanophyta, green algae, diatoms and chrysophyta and many other categories. There are many kinds of microalgae, up to more than 20,000 kinds. The accurate identification and classification of such a large number of species is a huge challenge we face. At this stage, the species identification of marine microalgae mainly depends on the observation of its morphology under a microscope. Microalgae, however, are small in shape and greatly affected by the environment, and have different morphological characteristics in different life cycles. Morphological identification is greatly affected by subjective fa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12Q1/6869C12Q1/04C12N15/11C40B50/06
CPCC12Q1/6895C12Q1/6869C40B50/06C12Q2531/113C12Q2535/122
Inventor 李亚军邓晓东
Owner INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI
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