Biomarker analysis method based on multiple immunohistochemical technologies and application thereof

A biomarker and immunohistochemical technology, applied in the field of biological detection and analysis, can solve problems such as the inability to explain the spatial relationship of tumor cells well, and achieve the effect of reducing complicated work.

Active Publication Date: 2021-09-28
深圳裕泰抗原科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] However, current studies on immuno-oncology focus on the role of specific immune cell subsets in their spatial environment, for example, through multiplex imaging, mass cytometry, and single-cell RNA sequencing, but they are not fully developed. Using information such as spatial coordinate data, the spatial relationship between tumor cells and surrounding elements cannot be well explained

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  • Biomarker analysis method based on multiple immunohistochemical technologies and application thereof
  • Biomarker analysis method based on multiple immunohistochemical technologies and application thereof
  • Biomarker analysis method based on multiple immunohistochemical technologies and application thereof

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Embodiment 1

[0075] Sample used in this embodiment is a clinical patient (Patient1) tumor tissue sample 1 case of lung cancer in laboratory testing. 7 the completed multi-color MOTiF immunohistochemical staining of tissue samples of tumor patients (e.g., for lung cancer TM PD-1 / PD-L1 Panel kit, comprising a targeting FoxP3, PD-L1, PanCK, PD-1, CD8, 6 antibodies for CD68), for the labeling fluorescent-positive cells, and through the entire film system VECTRA POLARIS Get scanning microscopy immunohistochemistry panorama. Phenochart by selecting the target area (tumor inner cells containing, the boundary region of the tumor cells and stromal cells), introduced inForm system, using official lung algorithm predefined rule-based classification acquired morphological and phenotypic changes, export data for biological marker analysis and calculation.

[0076] Biomarker analysis following steps:

[0077] 1. Read Data: Import "* cell_seg_data.txt" data to RStudio;

[0078] 2. Data extraction: extract ...

Embodiment 2

[0089] Sample used in this embodiment is (Patient2) tumor tissue sample in another one embodiment the clinical laboratory testing of lung cancer patients. Completed the patient tissue tumor sample multiple immunohistochemical staining (color 6 7 standard: FoxP3, PD-L1, PanCK, PD-1, CD8, CD68), and after VECTRA POLARIS slide scanning system for full acquisition Immunohistochemistry microscopic panorama. Phenochart by selecting the target area (tumor inner cells containing, the boundary region of the tumor cells and stromal cells), introduced into the system inForm, lung using a predefined algorithm official rules are organized based on morphological classification and typing of cells derived biological data for marker analysis and calculation.

[0090] Biomarker analysis following steps:

[0091] 1. Read Data: Import "* cell_seg_data.txt" data to RStudio;

[0092]2. Extract data: Extract critical information columns (including: organizational morphological classification columns, c...

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Abstract

The invention provides a biomarker analysis method based on multiple immunohistochemical technologies and application thereof. According to the biomarker analysis method, histomorphology, cell phenotype and corresponding space coordinate information of a tumor tissue sample of an image recognition system are utilized, and the tumor immune microenvironment is automatically analyzed through two biomarkers including double-positive cell scoring and tumor-immunosuppression relative distance ratio. The invention also provides a device and equipment for implementing the method, and a corresponding computer readable medium. By means of the method, the space connection state of the tumor immune microenvironment can be more accurately and efficiently represented, the tumor immune microenvironment related indexes can more effectively evaluate the immune treatment effect, and the requirements for tumor immune microenvironment analysis through multiple immunohistochemical technologies in clinical pathological work and scientific research are met.

Description

Technical field [0001] The present invention relates to a biological detection and analysis, and in particular relates to a method of analyzing a biomarker and its application based on multiple immunohistochemical techniques. Background technique [0002] Immunohistochemistry (Immunohistochemistry), also known as immunohistochemistry, the tissue distribution of which is an antigen (or antibody) is carried out in situ detection of tissues and cells with a specific antibody (or antigen) markers. As a mature, reliable, economical and strong and widely used technology, currently in clinical pathology and is widely used in scientific research, select a source for tumor tissue to determine, assess the risk of cancer, treatment, and various experiments observed tissue distribution and expression levels of cell antibody (or antigen) have an irreplaceable role. Immunohistochemical typically include a conventional immunohistochemistry and multiple immunohistochemistry: [0003] Conventiona...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G16B40/00
CPCG16B40/00
Inventor 李晓婷金皓玄王佳茜苏小凡
Owner 深圳裕泰抗原科技有限公司
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