Extraction process of nannochloropsis oculata essential oil

A technique of Nannochloropsis essential oil and an extraction process, which is applied in the field of Nannochloropsis, can solve problems such as affecting the smell of essential oils, and achieves the effects of pure aroma, high purity, and improved release degree

Active Publication Date: 2021-09-28
RIZHAO POLYTECHNIC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the fishy smell of Nannochloropsis, direct extraction of essential oils will obviously affect the smell of essential oils, making it difficult to be widely accepted. Therefore, improving the extraction method of Nannochloropsis essential oils to improve its odor quality is beneficial to Nannochloropsis. Important for essential oil extraction

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A kind of extraction technology of Nannochloropsis essential oil, concrete process is as follows:

[0028] (1) Pectinase treatment: mix Nannochloropsis with water, the weight ratio of Nannochloropsis to water is 30:100; stir to disperse Nannochloropsis to obtain a mixed solution; adjust the pH of the mixed solution 4.1, then add pectinase 4.1u / g to it, the enzymolysis temperature is 38°C, and maintain for 4.4h to obtain the enzymolysis solution;

[0029] (2) Collect algae: centrifuge the enzymatic hydrolyzate in step (1), collect the solid, and then wash the solid three times to obtain the algae; through centrifugation, the cell structure of Nannochloropsis is not destroyed. The colloid after enzymatic hydrolysis is filtered to avoid the impact of the colloid on the smell of essential oils;

[0030] (3) Juice extraction: use the freeze-thaw method to freeze and thaw the algae body three times, and then press the algae body to obtain the algae liquid; the process of the...

Embodiment 2

[0035] A kind of extraction technology of Nannochloropsis essential oil, concrete process is as follows:

[0036] (1) Pectinase treatment: mix Nannochloropsis with water, the weight ratio of Nannochloropsis to water is 20:100; stir to disperse Nannochloropsis to obtain a mixed solution; adjust the pH of the mixed solution 4.2, then add pectinase 3.5u / g to it, enzymolysis temperature is 35°C, keep for 4h to obtain enzymolysis solution;

[0037] (2) Collect algae: centrifuge the enzymatic hydrolyzate in step (1), collect the solid, and then wash the solid three times to obtain the algae; through centrifugation, the cell structure of Nannochloropsis is not destroyed. The colloid after enzymatic hydrolysis is filtered to avoid the impact of the colloid on the smell of essential oils;

[0038] (3) Juice extraction: use the freeze-thaw method to freeze and thaw the algae body three times, and then press the algae body to obtain the algae liquid; the process of the freeze-thaw metho...

Embodiment 3

[0043] A kind of extraction technology of Nannochloropsis essential oil, concrete process is as follows:

[0044] (1) Pectinase treatment: mix Nannochloropsis with water, the weight ratio of Nannochloropsis to water is 40:100; stir to disperse Nannochloropsis to obtain a mixed solution; adjust the pH of the mixed solution 4.3, then add pectinase 4.3u / g to it, the enzymolysis temperature is 40°C, and keep for 5h to obtain the enzymolysis solution;

[0045] (2) Collect algae: centrifuge the enzymatic hydrolyzate in step (1), collect the solid, and then wash the solid three times to obtain the algae; through centrifugation, the cell structure of Nannochloropsis is not destroyed. The colloid after enzymatic hydrolysis is filtered to avoid the impact of the colloid on the smell of essential oils;

[0046] (3) Juice extraction: use the freeze-thaw method to freeze and thaw the algae body three times, and then press the algae body to obtain the algae liquid; the process of the freez...

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PUM

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Abstract

The invention provides an extraction process of nannochloropsis oculata essential oil. The extraction process comprises the steps of pectinase treatment, thallus collection, juice extraction and distillation. In the pectinase treatment process, the addition amount of pectinase is 3.5-4.3 u/g, the enzymolysis temperature is 35-40 DEG C, and the condition is maintained for 4-5 hours. The extraction process has the advantages of being easy to operate, low in cost and suitable for wide popularization. The essential oil obtained by the extraction process has the advantages of no fishy smell and light and pleasant smell.

Description

technical field [0001] The invention relates to the technical field of Nannochloropsis, in particular to an extraction process of Nannochloropsis essential oil. Background technique [0002] Nannochloropsis, as a single-celled algae whose cell surface is covered with colloid, is rich in various minerals, lipids and proteins. With the increase of people's understanding of Nannochloropsis, the attention paid to its performance is also getting higher and higher. [0003] Essential oil is a general term for volatile aroma-containing substances, including acids, alcohols, lipids, hydrocarbons and other compounds, which exist in the roots, stems, leaves, flowers and seeds of plants, and are generally found in animals. in secretions. [0004] Nannochloropsis essential oil is rich in aroma components, and because Nannochloropsis is a healthy raw material resource, the application of the essential oil extracted from Nannochloropsis as food seasoning is gradually increasing. Howeve...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B9/02
CPCC11B9/02C11B9/027C11B9/025
Inventor 赵璐胡晓文张新明
Owner RIZHAO POLYTECHNIC
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