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Abamectin coloring agent and preparation and flaking method thereof

A technology of abamectin and preparation method, applied in the biological field, can solve the problems of long dyeing time and difficulty in observing and distinguishing the dead and alive of infecting bacteria, and achieve the effects of reducing production time, saving time, and meeting production needs

Active Publication Date: 2021-09-17
DAQING JEFENE BIO CHEM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The present invention is to overcome the traditional use of a single staining agent in the background technology to carry out staining of abamectin fermented liquid for a long time. The film made with this abamectin staining agent not only has clear observation of the producing bacteria, but also clearly distinguishes the color of the dead and living bacteria of the infecting bacteria

Method used

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  • Abamectin coloring agent and preparation and flaking method thereof
  • Abamectin coloring agent and preparation and flaking method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The preparation method of this Abamectin staining agent comprises the following steps:

[0025] 1) Preparation of liquid A: First weigh 1g of methylene blue and put it into the container, then add 30mL of 95% alcohol and stir well, then add distilled water to 300mL.

[0026] 2) Preparation of liquid B: first weigh 1g of basic fuchsin, add 20mL of 95% alcohol, and mix well. Weigh 5g of phenol and add 80~150mL of distilled water and mix well. Mix the phenol solution with basic fuchsin and mix thoroughly.

[0027] 3) Preparation of liquid A and liquid B mixture: add 4mL of liquid B to every 70mL of liquid A, mix well, drop the filter paper drop by drop to observe the color diffusion, the inner ring is blue, the outer ring is red, fine-tune A and B depending on the color liquid ratio.

[0028] The preparation method of this Abamectin staining agent comprises the following steps:

[0029] Stir the abamectin fermentation broth evenly, take 1 drop onto the glass slide, sma...

Embodiment 2

[0031] The preparation method of this Abamectin staining agent comprises the following steps:

[0032] 1) Preparation of liquid A: first weigh 1g of methylene blue and put it into the container, then add 60mL of 95% alcohol and stir evenly, then add distilled water to 600mL.

[0033] 2) Preparation of liquid B: first weigh 1g of basic fuchsin, add 50mL of 95% alcohol, and mix well. Weigh 10g of phenol and add 150mL of distilled water and mix well. Mix the phenol solution with basic fuchsin and mix thoroughly.

[0034] 3) Preparation of liquid A and liquid B mixture: add 10mL of liquid B to every 70mL of A liquid, mix well, drop on filter paper to observe the color diffusion, the inner ring is blue, the outer ring is red, fine-tune A and B depending on the color liquid ratio.

[0035] The preparation method of this Abamectin staining agent comprises the following steps:

[0036] Stir the abamectin fermentation broth evenly, take 1 drop onto the glass slide, smash the slide ...

Embodiment 3

[0038] The preparation method of this Abamectin staining agent comprises the following steps:

[0039] 1) Preparation of liquid A: First weigh 1g of methylene blue and put it into the container, then add 50mL of 95% alcohol and stir evenly, then add distilled water to 500mL.

[0040] 2) Preparation of liquid B: first weigh 1g of basic fuchsin, add 30mL of 95% alcohol, and mix well. Weigh 8g of phenol and add 100mL of distilled water and mix well. Mix the phenol solution with basic fuchsin and mix thoroughly.

[0041] 3) Preparation of liquid A and liquid B mixture: Add 7mL of liquid B to every 70mL of liquid A, mix well, drop the filter paper to observe the color diffusion, the inner circle is blue, the outer circle is red, fine-tune A and B depending on the color liquid ratio.

[0042] The preparation method of this Abamectin staining agent comprises the following steps:

[0043] Stir the abamectin fermentation broth evenly, take 1 drop onto the glass slide, smash the sli...

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Abstract

The invention relates to an abamectin coloring agent and a preparation and flaking method thereof. The problems of long coloring time and difficulty in observation when a single coloring agent is used for coloring the abamectin fermentation liquor in the prior art are mainly solved. The coloring agentis characterized in that the formula of the coloring agent comprises methylene blue, basic fuchsin, phenol, alcohol and distilled water. The preparation method of the coloring agent comprises the following steps of preparing a solution A: putting methylene blue into a container, adding 95% alcohol, uniformly stirring, and adding distilled water, preparing a solution B, namely adding the basic fuchsin into 95% alcohol, and uniformly mixing, adding distilled water into phenol and uniformly mixing, uniformly mixing the prepared phenol solution with the prepared basic fuchsin solution, adding the solution B into the solution A, uniformly mixing, dripping a drop of filter paper to observe the color diffusion condition with the inner circle being blue and the outer circle being red, and finely adjusting the ratio of the solution A to the solution B according to the color condition. According to a tablet prepared from the abamectin coloring agent, not only production bacteria are clearly observed, but also colors of dead and living bacteria of infection bacteria are obviously distinguished.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a preparation and film-making method of an abamectin staining agent. Background technique [0002] When the traditional melanin staining method using a single staining agent is used to stain the abamectin fermentation broth, the staining time is long; the use of alcohol lamps to fix the slices is not safe enough; the cost of decolorization with hydrochloric acid alcohol is high and the time is long, and only one time A single film, with a small quantity, cannot meet the needs of mass production, and it is difficult to distinguish between live and dead infested bacteria during observation. Contents of the invention [0003] The present invention is to overcome the traditional use of a single staining agent in the background technology to carry out staining of abamectin fermented liquid for a long time. The film made by using the abamectin staining agent not only has clear observati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/30G01N1/44G01N21/84
CPCG01N1/30G01N1/44G01N21/84G01N2001/302Y02A50/30
Inventor 李杰
Owner DAQING JEFENE BIO CHEM
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