A method for editing the rice giant embryo gene ge to cultivate giant embryo japonica varieties

Abstract

The invention discloses a method for editing rice giant embryo gene GE to cultivate giant embryo japonica rice varieties. The present invention uses the CRISPR-Cas9 multi-target system to control the size of rice embryos GE The megaembryo gene is edited at a fixed point, and the japonica rice genetic transformation is carried out to obtain the megaembryo transgenic plant, and the detection of the transgene insertion fragment in the offspring can shorten the breeding cycle and quickly obtain a variety of japonica rice varieties with large and small embryos without the insertion of the transgene fragment. same embryo size the ge The GABA content of the brown rice of the genotype mutants is also different, and the GABA content also increases with the growth of the embryo. Rice varieties with different embryo weights can satisfy the extraction of different GABA contents to meet the needs of different consumer groups. Therefore, the present invention can provide an efficient gene knockout method and a method for cultivating multiple giant embryo japonica rice varieties for production applications with different GABA content requirements.

Description

technical field [0001] The invention relates to the field of plant transgenic technology and crop genetic breeding, in particular to a method for editing rice giant embryo genes GE A method for cultivating giant-embryo japonica rice varieties; in particular, a method for editing rice using CRISPR-Cas9 multi-target technology GE genetically acquired variety GE The allele type mutant produces a variety of giant embryo materials whose seed embryos are larger than the wild type, and a breeding method for cultivating multiple giant embryo japonica rice varieties without insertion of transgene segments. Background technique [0002] Rice is an important food crop in my country and the staple food of more than 50% of the population in China. In recent years, with the improvement of people's living standards, people have higher pursuit of rice nutritional quality. Functional rice refers to rice that is rich in some physiologically active substances in addition to the nutritional c...

AI Technical Summary

Problems solved by technology

At present, giant embryo rice is mainly obtained by mutagenesis of the gene and hybrid breeding technology. However, these breeding methods require high cost, high labor intensity and long breeding cycle, and the size distribution of the obtained giant embryo rice seed embryos is limited.

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