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Bacterial carrier used for photodynamic therapy, and preparation method and application of bacterial carrier

A photodynamic therapy and carrier technology, which is applied in the directions of non-active ingredients medical preparations, medical preparations containing active ingredients, pharmaceutical formulas, etc. problem, to achieve the effect of increasing intake, improving treatment effect, and increasing sensitivity

Pending Publication Date: 2021-09-17
FUJIAN INST OF RES ON THE STRUCTURE OF MATTER CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on phthalocyanine mostly stays on the modification of phthalocyanine molecules, and the modification of targeting groups targets specific tumors. The repulsion prevents the drug from entering the tumor, greatly reducing the effect of the drug

Method used

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  • Bacterial carrier used for photodynamic therapy, and preparation method and application of bacterial carrier
  • Bacterial carrier used for photodynamic therapy, and preparation method and application of bacterial carrier
  • Bacterial carrier used for photodynamic therapy, and preparation method and application of bacterial carrier

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Embodiment 1

[0043] 1. The activation of Escherichia coli DH5α, its culture method is as follows:

[0044] (1) Prepare LB medium: 1g Trypton, 0.5g Yeast Extract, 1g NaCl dissolved in 100ml water, pH 7.0, sterilized at 121°C for 20min.

[0045] (2) Take out the frozen glycerol bacteria, inoculate them into 2ml of LB medium for activation, and culture them on a shaker (220rpm) at 37°C overnight. Then take 100 μl of the activated bacterial solution and inoculate it into 10 ml LB medium (1% inoculation), shake it at 37° C. (220 rpm) for 4 hours. Take 200 μl of bacterial liquid and read the OD600 absorption value with a microplate reader, and the value is about 0.6-0.8, and set aside.

[0046] 2. The preparation of bacterial carrier, its concrete method is as follows:

[0047] A phthalocyanine ZnPc-IR710 (compound represented by formula I) with four positive charges was selected, and prepared into a solution with a concentration of 2 mM in DMSO for use.

[0048] Dilute the ZnPc-IR710 solutio...

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Abstract

The invention discloses a bacterial carrier used for photodynamic therapy, and a preparation method and application of the bacterial carrier. Non-pathogenic Escherichia coli is used as a carrier to load a photosensitizer, a photodynamic therapy method is combined to realize the photodynamic therapy of tumor and realize delivery of the photosensitizer, the photosensitizer intake of tumor cells is significantly improved, the sensitivity of the focus to the photodynamic therapy method is increased, a treatment effect can be obviously improved, and the biological safety is good.

Description

technical field [0001] The invention relates to the field of bacterial drug loading, and more specifically relates to a bacterial carrier for photodynamic therapy and its preparation method and application. Background technique [0002] Since Coley discovered that bacteria can treat tumors more than 100 years ago, bacterial treatment of tumors has become a research hotspot in recent years, and breakthroughs have been made. The hypotheses of the tumor chemotaxis mechanism of bacteria can be summarized into three points: (1) The immune escape mechanism caused by the tumor site provides a shelter for the bacteria; (2) The acidic environment of the tumor and its surrounding tissues is suitable for the growth of bacteria; (3) The hypoxic environment of the tumor site and the core necrosis area, these conditions provide an ideal living environment for bacteria. At present, most of the research on bacterial therapy is at the level of genetic modification. Bacterial genetic modific...

Claims

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Application Information

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IPC IPC(8): A61K47/46A61K41/00A61P35/00
CPCA61K47/46A61K41/0071A61P35/00Y02A50/30
Inventor 陈卓戴涛胡萍潘小宏陈锦灿张蕾
Owner FUJIAN INST OF RES ON THE STRUCTURE OF MATTER CHINESE ACAD OF SCI
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