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Lactobacillus plantarum SHY130 and application thereof in relief of diabetes

A Lactobacillus plantarum, diabetes technology, applied in the field of microorganisms, can solve the problem of less research on the mechanism of action

Active Publication Date: 2021-09-14
重庆誉研生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, less research has been done on the mechanism of action of probiotics on the gut-island axis in diabetes

Method used

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  • Lactobacillus plantarum SHY130 and application thereof in relief of diabetes
  • Lactobacillus plantarum SHY130 and application thereof in relief of diabetes
  • Lactobacillus plantarum SHY130 and application thereof in relief of diabetes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1, Isolation and identification of Lactobacillus plantarum SHY130

[0041] 1 Experimental materials

[0042] Sichuan Hongyuan traditional fermented yak yogurt.

[0043] 2 Experimental methods

[0044] 2.1 Isolation and purification of lactic acid bacteria

[0045] The retrieved yak yoghurt was diluted 10 times, and then diluted to 10 -6 . Choose 4 appropriate dilutions (dilution is 10 -3 、10 -4 、10 -5 、10 -6) respectively spread 100 μL on the MRS solid plate medium, and after culturing at 37°C for 48 hours, single colonies with different shapes were selected to isolate the strains by streaking on the plate. The above steps were repeated until a purified strain was obtained, and the morphology was observed by Gram staining.

[0046] 2.2PCR amplification of 16S rDNA sequence

[0047] The DNA of the purified strains was extracted using a bacterial genomic DNA extraction kit. Use 25 μL reaction system for PCR amplification: template DNA 1 μL, upstream pri...

Embodiment 2

[0071] Example 2. The alleviating effect of Lactobacillus plantarum SHY130 on type Ⅱ diabetes mellitus in mice through the intestinal island axis

[0072] 1 Experimental materials

[0073] The experimental strain is Lactobacillus plantarum SHY130 (Lactobacillus plantarum SHY130), and the preservation number is CCTCC NO: M2021372.

[0074] The experimental animals were 4-week-old male C57BL / 6J mice, which were purchased from the Experimental Animal Center of Chongqing Medical University. Raise them in a standardized laboratory at room temperature 22±2°C, relative humidity 50±5%, 12h light / 12h dark, and start the experiment after one week of adaptive feeding.

[0075] 2 Experimental methods

[0076] 2.1 Grouping and handling of experimental animals

[0077] The mice were randomly divided into three groups: normal group (NC), diabetes group (D), and Lactobacillus plantarum SHY130 (SHY130), with 8 mice in each group. The experimental period was 16 weeks, and the animals in eac...

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Abstract

The invention discloses lactobacillus plantarum SHY130 which is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number is CCTCC NO: M 2021372. The invention discloses a microbial agent. An active component of the microbial agent comprises the lactobacillus plantarum SHY130. The invention further discloses application of the lactobacillus plantarum SHY130 or the microbial agent in preparation of products for preventing and / or relieving and / or treating diabetes. The invention also provides application of the lactobacillus plantarum SHY130 in preparation of foods, food additives, health care products or medicines as functional probiotics. According to the lactobacillus plantarum SHY130 and the application thereof, an action mechanism of the probiotics on regulating an intestinal island axis is explored, the application range of the lactobacillus plantarum SHY130 is expanded, the utilization value of the lactobacillus plantarum SHY130 is improved, and a new hope is brought to treatment of type II diabetes.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a strain of Lactobacillus plantarum SHY130 and its application for alleviating diabetes. Background technique [0002] Diabetes has become one of the most serious and critical public health problems facing the world in the 21st century. According to statistics from the International Diabetes Federation in 2019, about 463 million adults worldwide suffer from diabetes, and it is estimated that by 2045, the number of people with diabetes will reach 700 million. Type Ⅱ diabetes accounts for more than 90% of diabetic patients, mainly manifested as insulin resistance and relative lack of insulin caused by impaired function of pancreatic beta cells. Patients with type 2 diabetes have a significantly increased risk of cardiovascular disease (CVD), retinopathy, neuropathy, and renal failure, which can even be life-threatening in severe cases. The study found that patients with pa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/747A23L33/135A61P3/10C12R1/25
CPCA61K35/747A23L33/135A61P3/10A23V2002/00A23V2400/169A23V2200/328Y02A50/30
Inventor 索化夷
Owner 重庆誉研生物科技有限公司
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