Recycling application of 5 '-AMP and 5'-UMP in nucleotide chromatographic separation

A nucleotide and alkali technology, applied in chemical instruments and methods, preparation of sugar derivatives, sugar derivatives, etc., can solve the problems of large water consumption, affecting the yield of separation, and low purity of nucleotide products.

Pending Publication Date: 2021-09-14
NANTONG QIUZHIYOU BIOSCI & BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] During the separation process of nucleotide chromatography, 5'-AMP and 5'-UMP have about 20%-30% cross-mixing together. If this part is discarded, it will affect the yield of separation. If it is mixed with 5'-AMP or 5'- Crystallization in UMP will affect the quality of the product; and the existing positive column technology is to separate the four nucleotides, and the elution method is generally to collect the four nucleotides by elution with a large amount of water, so that not only the water consumption is very Large, and there is crossover between received and nucleotides, and the purity of the obtained nucleotide products is low

Method used

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  • Recycling application of 5 '-AMP and 5'-UMP in nucleotide chromatographic separation
  • Recycling application of 5 '-AMP and 5'-UMP in nucleotide chromatographic separation
  • Recycling application of 5 '-AMP and 5'-UMP in nucleotide chromatographic separation

Examples

Experimental program
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Effect test

Embodiment 1

[0029] see Figure 1-2 , a recovery application of 5'-AMP and 5'-UMP in the chromatographic separation of nucleotides, comprising the following steps:

[0030] S1. Regeneration of cation resin: regenerate the cation resin with alkali and acid, wash with water to pH=3.0-4.0, wash off the impurities on the resin, charge the resin, and set aside;

[0031] S2, AMP, UMP mixed sample loading: Dilute the mixture of AMP and UMP to a concentration of 20-30g / L, adjust the pH=2.0-2.5, load the sample, AMP is adsorbed by the positive column, and UMP flows out directly to the carbon column without being adsorbed collection and adsorption;

[0032] S3. Washing with water: After loading the samples, AMP and UMP were washed with 4 times the column volume respectively;

[0033] S4. Elution: AMP is eluted with 2% NaCl; UMP is eluted with alkali ethanol;

[0034] S5. Concentrated crystallization: the eluted AMP and UMP are concentrated and crystallized to obtain finished AMP and UMP.

Embodiment 2

[0036] see figure 1 , 3 , a recovery application of 5'-AMP and 5'-UMP in the chromatographic separation of nucleotides, comprising the following steps:

[0037] S1. Regeneration of cation resin: regenerate the cation resin with alkali and acid, wash with water to pH=3.0-4.0, wash off the impurities on the resin, charge the resin, and set aside;

[0038] S2, AMP, UMP mixed sample loading: Dilute the mixture of AMP and UMP to a concentration of 20-30g / L, adjust the pH=2.0-2.5, load the sample, AMP is adsorbed by the positive column, and UMP flows out directly to the carbon column without being adsorbed collection and adsorption;

[0039] S3. Washing with water: After loading the samples, AMP and UMP were washed with 4 times the column volume respectively;

[0040] S4. Elution: AMP is eluted with 3% NaCl; UMP is eluted with alkali ethanol;

[0041] S5. Concentrated crystallization: the eluted AMP and UMP are concentrated and crystallized to obtain finished AMP and UMP.

Embodiment 3

[0043] see figure 1 , 4 , a recovery application of 5'-AMP and 5'-UMP in the chromatographic separation of nucleotides, comprising the following steps:

[0044] S1. Regeneration of cation resin: regenerate the cation resin with alkali and acid, wash with water to pH=3.0-4.0, wash off the impurities on the resin, charge the resin, and set aside;

[0045] S2, AMP, UMP mixed sample loading: Dilute the mixture of AMP and UMP to a concentration of 20-30g / L, adjust the pH=2.0-2.5, load the sample, AMP is adsorbed by the positive column, and UMP flows out directly to the carbon column without being adsorbed collection and adsorption;

[0046] S3. Washing with water: After loading the samples, AMP and UMP were washed with 4 times the column volume respectively;

[0047] S4. Elution: AMP is eluted with 4% NaCl; UMP is eluted with alkali ethanol;

[0048] S5. Concentrated crystallization: the eluted AMP and UMP are concentrated and crystallized to obtain finished AMP and UMP.

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Abstract

The invention discloses a recycling application of 5 '-AMP and 5'-UMP in nucleotide chromatographic separation. The recycling application comprises the following steps: regenerating cation resin; mixing AMP and UMP and loading a sample; washing with water; performing elution; concentrating and crystallizing; and finally, obtaining finished products 5 '-AMP and 5'-UMP. According to the application, the structure difference of 5 '-AMP and 5'-UMP is utilized, that is, 5 '-UMP has no amino group, cannot carry positive charges during ionization under acidic conditions and cannot be exchanged by cation exchange resin, and AMP has amino group, carries positive charges during ionization under acidic conditions and can be exchanged by cation exchange resin, so that two nucleotides can be separated through cation exchange resin, the problem that part of AMP and UMP cannot be recycled or the quality is reduced after recycling is solved, and the total yield is increased by 20%-30%. Through exploration of elution of salts and alkali salts with different concentrations, a method for saving an elution solution and obtaining high-purity 5 '-AMP and 5'-UMP is found.

Description

technical field [0001] The invention relates to the technical field of nucleotide products, in particular to the recovery application of 5'-AMP and 5'-UMP in nucleotide chromatography separation. Background technique [0002] During the separation process of nucleotide chromatography, 5'-AMP and 5'-UMP have about 20%-30% cross-mixing together. If this part is discarded, it will affect the yield of separation. If it is mixed with 5'-AMP or 5'- Crystallization in UMP will affect the quality of the product; and the existing positive column technology is to separate the four nucleotides, and the elution method is generally to collect the four nucleotides by elution with a large amount of water, so that not only the water consumption is very Large, and there is crossover between received and nucleotides, and the purity of the obtained nucleotide products is low. Contents of the invention [0003] The object of the present invention is to provide a recovery application of 5'-AM...

Claims

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Application Information

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IPC IPC(8): C07H1/06C07H19/20C07H19/10
CPCC07H1/06C07H19/20C07H19/10
Inventor 徐浩陈修足邱蔚然邱志云
Owner NANTONG QIUZHIYOU BIOSCI & BIOTECH
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