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Trichogramma dendrolimus natural enemy attractant

A technology of pine caterpillar Trichogramma and attractant, which is applied in the directions of attracting pests, biocides, animal repellents, etc.

Active Publication Date: 2021-09-14
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] As far as the pear borer is concerned, there is no research and development of attractants for the natural enemy of the pear borer, the pine caterpillar Trichogramma

Method used

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  • Trichogramma dendrolimus natural enemy attractant
  • Trichogramma dendrolimus natural enemy attractant
  • Trichogramma dendrolimus natural enemy attractant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1: a kind of pine caterpillar Trichogramma attractant:

[0038] Dissolve 5 mg of methyl salicylate, 5 mg of phenylacetaldehyde, 5 mg of α-pinene, and 5 mg of tricosane in 980 mg of n-hexane.

[0039] Experiment 1. Take 20 mg of the Trichogramma pine caterpillar attractant obtained in Example 1 and evenly drop it on a filter paper of 2 cm×1 cm to serve as an odor source.

[0040] The trunk and two arms of the Y-shaped olfactometer are all 10 cm long, 1 cm in diameter, and the angle between the two arms is 75°. Each part of the olfactometer is connected in turn by medical rubber tubes. The gas is blown out by the air pump, passes through the activated carbon, the distilled water bottle, the flow meter, the smell bottle in turn, and then enters the two arms of the Y-shaped olfactometer. The flow meter airflow is about 300ml / min.

[0041] Take a mated female Trichogramma with a brush each time, place it at the entrance of the trunk of the Y-shaped olfactometer...

Embodiment 2

[0074] Embodiment 2: the attractant of following formula is set respectively:

[0075] Attractant 1: Dissolve 5 mg methyl salicylate, 0.5 mg phenylacetaldehyde, 5 mg α-pinene, and 5 mg tricosane in 980 mg n-hexane.

[0076] Attractant 2: Dissolve 5 mg methyl salicylate, 50 mg phenylacetaldehyde, 5 mg α-pinene, and 5 mg tricosane in 980 mg n-hexane.

[0077] Attractant 3: 5 mg methyl salicylate, 5 mg phenylacetaldehyde, 0.5 mg α-pinene, 5 mg tricosane were dissolved in 980 mg n-hexane.

[0078] Attractant 4: 5 mg of methyl salicylate, 5 mg of phenylacetaldehyde, 3 mg of α-pinene, and 5 mg of tricosane were dissolved in 980 mg of n-hexane.

[0079] Attractant 5: 5 mg of methyl salicylate, 5 mg of phenylacetaldehyde, 6 mg of α-pinene, and 5 mg of tricosane were dissolved in 980 mg of n-hexane.

[0080] Attractant 6: 5 mg of methyl salicylate, 5 mg of phenylacetaldehyde, 5 mg of α-pinene, and 0.05 mg of tricosane were dissolved in 980 mg of n-hexane.

[0081] Attractant 7: 5 mg...

Embodiment 3

[0088] Embodiment 3, attractant is to the field attracting action of pine caterpillar Trichogramma

[0089] Field experiments were used to test the actual attractant effect of the better attractant formula on Trichogramma pine caterpillar. Each compound was compounded according to the mass ratio in Table 5, and the attractant formula was made. Take 20 mg of the formula for each treatment, and add it dropwise into the rubber head lure carrier. After the solvent volatilizes, each formula compound is adsorbed in the rubber head lure core, and the valid period is about 15 days. Rubber head lures are used in conjunction with sticky boards, and are marked to facilitate the recording of the number of Trichogramma pine caterpillars obtained by sticking.

[0090] The field experiment consisted of 7 treatments (as shown in Table 5), each with 5 repetitions, and a total of 35 small treatments. Hang the Trichogramma egg card and formula treatment one day before the pine caterpillar Tri...

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Abstract

The invention discloses a trichogramma dendrolimus natural enemy attractant. The attractant is mainly composed of healthy plant volatile matter, pest-induced plant volatile matter and pest gonad / attached gland volatile matter. The healthy plant volatile matter is alpha-pinene, the pest-induced plant volatile matter is composed of methyl salicylate and phenylacetaldehyde, and the pest gonad / attached gland volatile matter is tricosane. On the basis of a chemical communication relationship among plants, pests and natural enemies, a compound formula with remarkable attraction activity on trichogramma dendrolimus is screened from a plurality of components such as the healthy plant volatile matter, the pest-induced plant volatile matter and the pest gonad / attached gland volatile matter; and the flying distance of trichogramma dendrolimus, the parasitism rate of grapholitha molesta and the biological control efficiency in the natural field environment are improved.

Description

technical field [0001] The invention relates to a Trichogramma pine caterpillar attractant for green prevention and control of the small pear borer. Background technique [0002] The small pear borer (Grapholitha molesta Busck, hereinafter referred to as Grapholitha Busck) belongs to the order Lepidoptera and the family Lepidoptera, and mainly harms the fruits and young shoots of drupe and pome fruit trees such as pear trees, peach trees, and apple trees. According to its hazard characteristics, Lixiao is also known as black plaster, peach tree heartworm, oriental fruit moth, etc. It not only has a serious overlapping of generations, but also has the characteristic of transferring hosts to parasitize. It is one of the fruit-boring pests that seriously affects the healthy development of the fruit industry, and brings a lot of economic losses to fruit production at home and abroad. [0003] At present, comprehensive control methods are adopted at home and abroad to prevent an...

Claims

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Application Information

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IPC IPC(8): A01N27/00A01N37/40A01N35/04A01P19/00A01M1/02
CPCA01N27/00A01N37/40A01N35/04A01M1/02A01M1/023A01M2200/012
Inventor 程敬丽梁敏敏王忠燕赵金浩
Owner ZHEJIANG UNIV
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