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A kind of perilla seed peptide capable of improving male sexual function and preparation method thereof

A technology of perilla seed peptide and sexual function, applied in the field of deep processing of perilla, can solve the problems of less reports and restricted deep processing of perilla, and achieve the effects of high product value, improvement of male sexual function, and widening of application scope.

Active Publication Date: 2022-08-09
CHINA NAT RES INST OF FOOD & FERMENTATION IND CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there have been some studies on the extraction of protein from perilla seed meal by using the principle of alkali-soluble acid precipitation, but there are few reports on the deep processing of protein and further research, which also limits the use of perilla as raw material. The research process of deep processing

Method used

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  • A kind of perilla seed peptide capable of improving male sexual function and preparation method thereof
  • A kind of perilla seed peptide capable of improving male sexual function and preparation method thereof
  • A kind of perilla seed peptide capable of improving male sexual function and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0071] The perilla seed peptide of the present embodiment is prepared according to the following method:

[0072] 1. Dissolve 100 g of perilla seed protein powder with a protein content of 80% in 1000 mL of water with a temperature of 50°C and stir to obtain a perilla seed protein homogenate;

[0073] 2. After adjusting the pH of the homogenate to 6.0 with 10% HCl solution, add 0.2 g of acid protease, 0.4 g of neutral protease, and 0.15 g of papain at the same time under the condition of 50 °C incubation, and then enzymatically hydrolyze for 3 hours under stirring. , boiled for 30min to inactivate the enzyme to obtain perilla seed peptide enzymatic hydrolysis solution;

[0074] 3. Centrifuge the perilla seed peptide enzymatic hydrolysis solution with a desktop centrifuge, and centrifuge at a centrifugal speed of 3000 rpm for 10 minutes to obtain about 800 mL of perilla seed peptide enzymatic hydrolysis centrifuge;

[0075] 4.1. At 50°C and the pressure difference between the ...

Embodiment 2

[0094] The perilla seed peptide of the present embodiment is prepared according to the following method:

[0095] 1. Dissolve 100kg of perilla seed protein powder with a protein content of 80% in an enzymolysis tank filled with 800L of pure water with a temperature of 52°C, stir and set the volume to 1.2m 3 , to obtain perilla seed protein homogenate;

[0096] 2. After adjusting the pH of the homogenate to 6.0 with 10% HCl solution, add 200 g of acid protease, 400 g of neutral protease, and 150 g of papain at the same time under the condition of 51 ℃ insulation, and use plate heat exchange after enzymolysis with stirring for 3 hours. After heating the enzymatic hydrolysis system with steam as the heat medium to inactivate the enzyme, the perilla seed peptide enzymatic hydrolysis solution is obtained, and the inactivation condition is 125°C for 20s;

[0097] 3. Centrifuge the perilla seed peptide enzymatic hydrolysis solution with a horizontal spiral decanter centrifuge at 348...

Embodiment 3

[0108] 1. Dissolve 100kg of perilla seed protein powder with a protein content of 80% in an enzymolysis tank filled with 800L of pure water with a temperature of 52°C, stir and set the volume to 1.2m 3 , to obtain perilla seed protein homogenate;

[0109] 2. After adjusting the pH of the homogenate to 6.0 with 10% HCl solution, add 320 g of acid protease, 530 g of neutral protease, and 200 g of papain at the same time under the condition of 51°C insulation, and use plate heat exchange after enzymatic hydrolysis under stirring for 3 hours. After heating the enzymatic hydrolysis system with steam as the heat medium to inactivate the enzyme, the perilla seed peptide enzymatic hydrolysis solution is obtained, and the inactivation condition is 125°C for 20s;

[0110] 3. Centrifuge the perilla seed peptide enzymatic hydrolysis solution with a horizontal spiral decanter centrifuge at 3480 rpm of the main motor and 2860 rpm of the auxiliary motor to obtain about 1050L of perilla seed ...

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Abstract

The invention provides a perilla seed peptide capable of improving male sexual function and a preparation method thereof. The first aspect of the present invention provides a perilla seed peptide capable of improving male sexual function, the composition of the perilla seed peptide at least includes peptide segments pER, AR and MR; based on the quality of the perilla seed peptide, the peptide segment The content of pER ≥ 10mg / 100g, the content of peptide AR ≥ 40mg / 100g, and the content of peptide MR ≥ 10mg / 100g. The perilla seed peptide containing the three peptide segments of pER, AR and MR provided by the present invention can increase the concentrations of testosterone (T), luteinizing hormone (LH) and follicle stimulating hormone (FSH) in serum, and can improve male sexual function effect.

Description

technical field [0001] The invention relates to a perilla seed peptide capable of improving male sexual function and a preparation method thereof, and relates to the technical field of perilla deep processing. Background technique [0002] Perilla frutescens (L.) Britt.) is a wild or planted annual herb of the Labiatae family in Asia, especially East Asia and Southeast Asia. Because Perilla frutescens has good edible and medicinal value, its stems and leaves (Perilla) and fruit (Perilla seeds) are included in the "Notice on Further Standardizing the Management of Health Food Raw Materials" and "List of Medicines and Food Homologous Products" issued by the Ministry of Health. [0003] Perilla seeds are rich in unsaturated fatty acids, especially polyunsaturated fatty acids, so perilla seeds are also recognized as a high-value and high-nutrition vegetable oil raw material. At present, the preparation process of using perilla seeds as raw materials to extract oils and fats is ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C07K1/36C07K1/34C07K1/24A23L33/18
CPCC12P21/06C07K1/36C07K1/34C07K1/24A23L33/18A23V2002/00A23V2200/30Y02P20/54
Inventor 蔡木易谷瑞增姜放姜盛郭颖董哲潘兴昌陈亮王雨辰魏颖刘艳王雨晴马勇马永庆徐亚光陆路刘文颖张海欣王憬李国明周明凌空毕园崔欣悦秦修远方磊
Owner CHINA NAT RES INST OF FOOD & FERMENTATION IND CO LTD
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