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Method for preparing N-acetylglucosamine fermentation clear liquid by using composite flocculant

A technology of glucose fermentation liquid and composite flocculant, which is applied in the field of bioengineering, can solve the problems of small bacteria, large amount of bacteria, and inability to filter the bacteria plate and frame, and achieve the effect of promoting flocculation, reducing dosage, and improving flocculation effect

Pending Publication Date: 2021-09-07
JIANGSU HEVI BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Also, due to the high-density fermentation of engineering bacteria with a large amount of bacteria and small bacteria, it must be flocculated before it can be filtered with a plate and frame. During the actual research process, it was found that neither a single inorganic flocculant nor an organic flocculant can flocculate the bacteria to achieve a perfect plate and frame filter. Therefore, our company has developed a kind of adding inorganic and organic composite flocculants, so that the high-density bacteria can be perfectly flocculated.

Method used

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  • Method for preparing N-acetylglucosamine fermentation clear liquid by using composite flocculant

Examples

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Effect test

example 1

[0038] Take high engineered bacteria density N- acetylglucosamine broth 40L, concentrated fermentation bacteria OD = 97, 85 ℃ jacket was heated to inactivate; open stirring to 450rpm, was added 200g (W / V) PFS, pH adjusted to 4.5, was added with stirring 160g 240g celite and activated carbon, after stirring for 10min, the stirring speed reduced to 110 rpm, at which time the solution was added 100ml 2% chitosan concentration, reduced stirring speed of 50 rpm uniformly stirred, while cooling after stopping stirring to 50 deg.] C, stationary for 30 min flocculation; then frame filter; whole broth had to be finished, for the top 30% of pure water, and dry plate and frame, the finally obtained clear N- acetylglucosamine fermentation liquid 45L, acetylglucosamine yield 99%, 7kg bacterial sludge cake, the moisture content of 68%.

example 2

[0040] Take high engineered bacteria density N- acetylglucosamine broth 340L, concentrated fermentation bacteria OD = 110, the jacket was heated to 95 deg.] C inactivated; open 300rpm stirring to inactivation, addition of 2.0kg PFS adjusted to pH 5.0 while stirring was added 4.0kg and 6.0kg activated carbon through celite. after stirring 10min, the stirring speed reduced to 110 rpm, at which time the solution concentration of 3% chitin 600ml, stirred for 10min after cooling to 50 ℃, the speed at 50rpm continued slow stirring, flocculation 60min, then plate and frame filter, filtration time 3h, the fermentation broth until all filters finished, the top 20% of the filter cake was washed with pure water, and dry plate and frame, the finally obtained fermentation liquid acetylglucosamine 370L, N- acetylglucosamine yield 98%; bacterial sludge filter cake was 79kg, 74% moisture content.

example 3

[0042] Take high engineered bacteria density N- acetylglucosamine broth a 4T, concentrated fermentation bacteria OD = 82, the jacket was heated to 95 deg.] C inactivated; inactivated broth after the flocculation tank 10t of hit, stirred open to the 50Hz, 20kg PFS was added to adjust pH to 4.5, while stirring, added 16kg 24kg celite and activated carbon, after stirring for 10min and the stirring speed reduced to 30Hz, chitin case 10L solution concentration of 2% was added, stirred for 10min cooled to 50 ℃, stirring was stopped flocculation 60min, then plate and frame filter; broth until all filters finished, the top 10% of the filter cake was washed with pure water, and dry plate and frame, the finally obtained N- acetylglucosamine fermentation liquid 3.7t, N- acetylglucosamine yield 98%; bacterial sludge cake 650kg, 68% moisture content.

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Abstract

The invention relates to a method for preparing N-acetylglucosamine fermentation clear liquid by using a composite flocculant, and belongs to the field of bioengineering. The method comprises the following specific steps of: inactivating N-acetylglucosamine fermentation liquid; adding an inorganic flocculant, and stirring to obtain N-acetylglucosamine fermentation liquid containing the inorganic flocculant; adding an adsorbent and a filter aid, stirring, adding a prepared organic polymer flocculant, stirring uniformly, and standing for flocculation for 30-60 minutes to obtain a flocculation liquid of the N-acetylglucosamine fermentation liquid; and performing plate-frame pressure filtration to obtain the N-acetylglucosamine fermentation clear liquid. The flocculation impurity removal process has an obvious effect, the clear liquid obtained after plate-frame filtration has high yield, the water content of mushroom dregs is low, and the use amount of the flocculant is reduced by composite flocculation. The process is simple to operate, has no three-waste emission, and is an environment-friendly, simple and efficient method for preparing the acetylglucosamine fermentation clear liquid.

Description

Technical field [0001] The present invention belongs to the technical field of biological engineering, in particular, relates to a composite flocculant Preparation of N- acetylglucosamine fermentation liquid method. Background technique [0002] N- acetylglucosamine formula C 8 Hide 15 NO 6 . As a white powder, soluble in water, it is the basic unit of the polysaccharide in a number of important biological cells, especially the highest content of the exoskeleton of crustaceans. It is important precursors for the synthesis Bifidobacterium factor, has a number of important physiological functions in vivo. For example, as chitin monomers. Has anti-inflammatory, anti-tumor, anti-oxidation effects, the clinical treatment of osteoarthritis, rheumatoid arthritis drugs, may be used as food antioxidants and diabetic infant food additives sweeteners, food, medicine and cosmetics and other fields are widely used. [0003] With the rapid development of genetic engineering and biological synt...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H5/06C07H1/06
CPCC07H5/06C07H1/06
Inventor 章文劼单志萍章金元
Owner JIANGSU HEVI BIOTECH CO LTD
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