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Microbacterium XL1 and application thereof in producing Levan fructan

A technology of microbacteria and fructans is applied in the field of bioengineering to achieve the effects of easy control of fermentation conditions, high carbon source utilization, and simple operation.

Active Publication Date: 2021-08-31
JIANGSU OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It can be seen that the carbon source utilization rate of levan fructan produced by the existing strains still needs to be improved

Method used

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  • Microbacterium XL1 and application thereof in producing Levan fructan
  • Microbacterium XL1 and application thereof in producing Levan fructan
  • Microbacterium XL1 and application thereof in producing Levan fructan

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Embodiment Construction

[0042] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. The following description of at least one exemplary embodiment is merely illustrative in nature and in no way taken as limiting the invention, its application or uses. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without creative efforts fall within the protection scope of the present invention.

[0043] The present invention will be described in detail below in conjunction with the accompanying drawings, so that those skilled in the art can easily understand the present invention.

[0044] The inoculum amount involved in the present invention all refers to the volume transferred into the seed...

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Abstract

The invention relates to the technical field of bioengineering, and particularly relates to a microbacterium XL1 and application thereof in producing Levan fructan. The invention provides a strain with a preservation number of CCTCC NO: M2021423, and discloses application of the strain in producing the Levan fructan. In addition, the invention further provides a specific method for screening the strain and culturing the strain to produce the Levan fructan. Through multiple tests, optimal culture conditions for the strain to produce the Levan fructan are provided, so that the carbon-source use efficiency for producing the Levan fructan exceeds 64%.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a microbacterium XL1 and its application in producing Levan fructan. Background technique [0002] Fructan is a homopolysaccharide composed of β-2,6 bonds and β-2,1 bonds connecting fructofuranose groups, and is a widely distributed biopolymer in nature. According to the different linkages, there are two types of fructans: inulin and levan. Inulin is connected by β-2,1 glycosidic bonds, usually comes from plants, and is used as a storage sugar in Asteraceae and Poaceae, so inulin-type fructans can be isolated from natural plants. Levan is a fructan composed of β-2,6 glycosidic bonds connecting D-fructofuranose residues as the main chain and some β-2,1 glycosidic bonds as branch points. It is usually derived from microorganisms, and the vast majority of Bacterial fructans are all levan-type fructans. [0003] Levan-type fructan has anti-tumor, anti-diabetic, hypolipidemi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P19/04C12R1/01
CPCC12N1/20C12P19/04
Inventor 徐淋香吴丹张岩殷先伟刘楠楠王震魏真徐海洋
Owner JIANGSU OCEAN UNIV
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