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Combined fermentation method of alcohol and succinic acid

A technology of ethanol fermentation and combined fermentation, applied in the direction of microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve the problem of high cost, achieve the effects of reducing raw material costs, improving carbon source utilization, and reducing costs

Active Publication Date: 2012-05-23
ANHUI BBCA FERMENTATION TECH ENG RES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, mainly through the recovery of CO 2 Production of liquid CO 2 , light calcium carbonate, fully degradable plastic - polypropylene carbonate resin, but the cost of recycling is relatively high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1 establishes ethanol fermentation system

[0022] The establishment of ethanol fermentation system comprises the following steps:

[0023] (1) plate culture

[0024] The composition of the plate medium is 3% glucose, 0.5% yeast extract, 0.5% peptone and 2% agar. Alcoholic yeast strains were inoculated into the medium, and cultured at 33°C for 28 hours.

[0025] (2) Shake flask seed culture

[0026] The composition of the shake flask seed medium is glucose 3%, yeast extract 0.5%, peptone 0.15%, corn steep liquor 0.5%, potassium dihydrogen phosphate 0.05%, magnesium sulfate 0.06%, and tap water.

[0027] The alcoholic yeast strain cultured on the plate was inoculated into the above-mentioned shake flask seed medium, and cultured at 33° C. for 30 hours, and the rotation speed of the shaker was 200 rpm.

[0028] (3) Seed jar seed culture

[0029] The composition of the seed medium for the seed pot is 5% of glucose, 0.5% of yeast extract, 0.05% of peptone, 0...

Embodiment 2

[0034] Embodiment 2 The succinic acid fermentation system combined with the ethanol fermentation system

[0035] The production method of succinic acid comprises the following steps:

[0036] (1) Plate culture: Inoculate the succinic acid-producing strain Actinobacillus succinate into tryptone soybean agar medium, and culture it anaerobically at 35° C. for 25 hours.

[0037] (2) Shake flask seed culture: the composition of shake flask seed culture medium is glucose 2%, yeast extract 0.4%, peptone 0.2%, corn steep liquor 0.3%, Na 2 CO 3 0.9%, NaH 2 PO 4 0.7%, K 2 HPO 4 1.0%, Na 2 HPO 4 0.2%, NaCl0.2%, tap water preparation. Inoculate the succinic acid bacterial species cultured on the plate into a serum bottle containing 200ml shake flask seed medium for shake flask culture, cultivate at 35°C for 15 hours, and the shaker speed is 180rpm.

[0038] (3) Seed tank culture: the composition of the seed tank seed medium is 2% glucose, 0.4% yeast extract, 0.2% peptone, 0.3% co...

Embodiment 3

[0042] Embodiment 3 The succinic acid fermentation system combined with the ethanol fermentation system

[0043] The production method of succinic acid comprises the following steps:

[0044] (1) Plate culture: Inoculate the succinic acid-producing strain Actinobacillus succinate into tryptone soybean agar medium, and culture it anaerobically at 35° C. for 27 hours.

[0045] (2) Shake flask seed culture: the composition of shake flask seed culture medium is glucose 2%, yeast extract 0.4%, peptone 0.2%, corn steep liquor 0.3%, Na 2 CO 3 0.9%, NaH 2 PO 4 0.7%, K 2 HPO 4 1.0%, Na 2 HPO 4 0.2%, NaCl0.2%, tap water preparation. Inoculate the succinic acid bacteria cultured on the plate into a serum bottle containing 200ml shake flask seed medium for shake flask culture, cultivate at 35°C for 17 hours, and the shaker speed is 180rpm.

[0046] (3) Seed tank culture: the composition of the seed tank seed medium is 2% glucose, 0.4% yeast extract, 0.2% peptone, 0.3% corn steep li...

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PUM

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Abstract

The invention relates to a combined fermentation method of alcohol and succinic acid. Alcohol is fermented and produced in an alcohol fermenting system, and succinic acid is fermented and produced in a succinic acid fermenting system; gas produced in the alcohol fermenting process is firstly treated, and then is led into a succinic acid fermenting and producing system. The method of the invention uses CO2 tail gas produced in the alcohol fermenting process in the succinic acid fermenting and producing process, which effectively reduces effect on environment due to CO2 discharging in the alcohol fermenting process, lowers tail treating cost in the alcohol fermenting process, greatly lowers carbonate adding amount in the succinic acid fermenting process and effectively lowers auxiliary material consumption for producing succinic acid.

Description

technical field [0001] The invention relates to a production method of ethanol and succinic acid, in particular to a combined fermentation method for producing ethanol and succinic acid. Background technique [0002] Succinic acid, also known as succinic acid, is a dibasic organic acid, which is an important C 4 Platform compounds, which can be used as intermediates in the synthesis of complex organic compounds, are widely used in pharmaceuticals, food processing, synthetic plastics, rubber, protective coatings, dyes and other industries. At present, more than 250 kinds of chemical products using benzene as raw materials can be obtained through the conversion of succinic acid, so the market demand for succinic acid continues to increase. [0003] There are two main methods for producing succinic acid: chemical synthesis and microbial fermentation. Due to the use of fossil raw materials and environmental pollution, the traditional chemical synthesis method has greatly restr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/06C12P7/46C12R1/19
CPCY02E50/17Y02E50/10
Inventor 李荣杰欧阳平凯薛培俭姜岷尚海涛
Owner ANHUI BBCA FERMENTATION TECH ENG RES
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