Combined fermentation method of alcohol and succinic acid
A technology of ethanol fermentation and combined fermentation, applied in the direction of microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve the problem of high cost, achieve the effects of reducing raw material costs, improving carbon source utilization, and reducing costs
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Embodiment 1
[0021] Embodiment 1 establishes ethanol fermentation system
[0022] The establishment of ethanol fermentation system comprises the following steps:
[0023] (1) plate culture
[0024] The composition of the plate medium is 3% glucose, 0.5% yeast extract, 0.5% peptone and 2% agar. Alcoholic yeast strains were inoculated into the medium, and cultured at 33°C for 28 hours.
[0025] (2) Shake flask seed culture
[0026] The composition of the shake flask seed medium is glucose 3%, yeast extract 0.5%, peptone 0.15%, corn steep liquor 0.5%, potassium dihydrogen phosphate 0.05%, magnesium sulfate 0.06%, and tap water.
[0027] The alcoholic yeast strain cultured on the plate was inoculated into the above-mentioned shake flask seed medium, and cultured at 33° C. for 30 hours, and the rotation speed of the shaker was 200 rpm.
[0028] (3) Seed jar seed culture
[0029] The composition of the seed medium for the seed pot is 5% of glucose, 0.5% of yeast extract, 0.05% of peptone, 0...
Embodiment 2
[0034] Embodiment 2 The succinic acid fermentation system combined with the ethanol fermentation system
[0035] The production method of succinic acid comprises the following steps:
[0036] (1) Plate culture: Inoculate the succinic acid-producing strain Actinobacillus succinate into tryptone soybean agar medium, and culture it anaerobically at 35° C. for 25 hours.
[0037] (2) Shake flask seed culture: the composition of shake flask seed culture medium is glucose 2%, yeast extract 0.4%, peptone 0.2%, corn steep liquor 0.3%, Na 2 CO 3 0.9%, NaH 2 PO 4 0.7%, K 2 HPO 4 1.0%, Na 2 HPO 4 0.2%, NaCl0.2%, tap water preparation. Inoculate the succinic acid bacterial species cultured on the plate into a serum bottle containing 200ml shake flask seed medium for shake flask culture, cultivate at 35°C for 15 hours, and the shaker speed is 180rpm.
[0038] (3) Seed tank culture: the composition of the seed tank seed medium is 2% glucose, 0.4% yeast extract, 0.2% peptone, 0.3% co...
Embodiment 3
[0042] Embodiment 3 The succinic acid fermentation system combined with the ethanol fermentation system
[0043] The production method of succinic acid comprises the following steps:
[0044] (1) Plate culture: Inoculate the succinic acid-producing strain Actinobacillus succinate into tryptone soybean agar medium, and culture it anaerobically at 35° C. for 27 hours.
[0045] (2) Shake flask seed culture: the composition of shake flask seed culture medium is glucose 2%, yeast extract 0.4%, peptone 0.2%, corn steep liquor 0.3%, Na 2 CO 3 0.9%, NaH 2 PO 4 0.7%, K 2 HPO 4 1.0%, Na 2 HPO 4 0.2%, NaCl0.2%, tap water preparation. Inoculate the succinic acid bacteria cultured on the plate into a serum bottle containing 200ml shake flask seed medium for shake flask culture, cultivate at 35°C for 17 hours, and the shaker speed is 180rpm.
[0046] (3) Seed tank culture: the composition of the seed tank seed medium is 2% glucose, 0.4% yeast extract, 0.2% peptone, 0.3% corn steep li...
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