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Application of combination of UP II antigen and UP II antibody in preparation of product for detecting bladder cancer

A bladder cancer and antigen technology, applied in the field of urogenital tumor detection products, can solve the problems of limited sample type, low sensitivity, poor repeatability, etc.

Pending Publication Date: 2021-08-24
深圳市龙华区中心医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are the following defects: First, NMP22 is a three-dimensional network structure protein involved in maintaining the function of the nucleus, which is not unique to the urothelium, and is released into the urine through apoptosis
The second is that the detection method used is the colloidal gold method, which is a semi-quantitative method. Compared with immunoluminescence, it has low accuracy, low sensitivity and low precision, and poor repeatability, so it cannot be used for automation.
Third, the sample type is limited to urine
[0009] In summary, the methods for diagnosing bladder cancer in the prior art all have defects

Method used

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  • Application of combination of UP II antigen and UP II antibody in preparation of product for detecting bladder cancer
  • Application of combination of UP II antigen and UP II antibody in preparation of product for detecting bladder cancer
  • Application of combination of UP II antigen and UP II antibody in preparation of product for detecting bladder cancer

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Experimental program
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Effect test

Embodiment 1

[0048] The kit for detecting bladder cancer in this embodiment is a kit for detecting bladder cancer by alkaline phosphatase chemiluminescence method. The kit for detecting bladder cancer comprises alkaline phosphatase-labeled UPII antibody, biotin-labeled monoclonal UPII antigen, streptavidin-coated magnetic bead suspension, luminescent substrate, standard and buffer , wherein, the concentration of the streptavidin-coated magnetic bead suspension is 0.1 mg / mL; the luminescent substrate is AMPPD; the standard substance has a concentration of 0 ng / mL, 0.5 ng / mL, The UPII antigen of 1ng / mL, 2ng / mL, 4ng / mL and 8ng / mL; The buffer solution includes buffer system, protein, surfactant and preservative, and the buffer system is a phosphate buffer solution with a pH value of 7.4, The protein is bovine serum albumin with a mass percentage of 0.1%, the surface activity is Tween 20 with a mass percentage of 0.05%, and the preservative is 0.05% by mass of ProClin300 and 0.01% by mass. nit...

Embodiment 2

[0059] The kit for detecting bladder cancer in this embodiment is a kit for detecting bladder cancer by alkaline phosphatase chemiluminescence method. The kit for detecting bladder cancer includes alkaline phosphatase-labeled UPII antibody, UPII antigen-coated magnetic bead suspension, a luminescent substrate, a standard and a buffer, wherein the luminescent substrate is AMPPD; Described standard product is the UPII antigen that concentration is 0ng / mL, 0.5ng / mL, 1ng / mL, 2ng / mL, 4ng / mL and 8ng / mL; Described buffer system is the phosphate buffer that pH value is 7.4, and described The protein is bovine serum albumin with a mass percentage of 0.1%, the surface activity is Tween 20 with a mass percentage of 0.05%, and the preservative is 0.05% by mass of ProClin300 and 0.01% by mass of sodium azide .

[0060] Using the kit to detect bladder cancer includes the following steps:

[0061] Step 1: Add 50 μL standard / sample and 50 μL alkaline phosphatase-labeled UPII antibody to a 9...

Embodiment 3

[0070] The kit for detecting bladder cancer in this embodiment is a kit for detecting bladder cancer by horseradish peroxidase chemiluminescence method. The kit for detecting bladder cancer comprises horseradish peroxidase-labeled UPII antibody, biotin-labeled monoclonal UPII antigen, streptavidin-coated magnetic bead suspension, luminescent substrate, standard and Buffer, wherein, the concentration of the streptavidin-coated magnetic bead suspension is 0.1mg / mL; the luminescent substrate is Lumi-Phos 530; the standard substance has a concentration of 0ng / mL, The UPII antigen of 0.5ng / mL, 1ng / mL, 2ng / mL, 4ng / mL and 8ng / mL; The buffer solution includes a buffer system, protein, surfactant and preservative, and the buffer system has a pH value of 7.4 Tris-HCl buffer solution, the protein is bovine serum albumin of 0.1% by mass percent, the surface activity is Tween 20 of 0.05% by mass percent, and the preservative is ProClin300 and 0.05% by mass percent of the preservative The ...

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Abstract

The invention discloses application of combination of UP II antigen and UP II antibody in preparation of a product for detecting bladder cancer, and belongs to the technical field of urogenital tumor detection products. Research finds that the combination of the UP II antigen and the UP II antibody can be used for preparing a product for detecting the bladder cancer, so that not only are new application fields of the UP II antigen and the UP II antibody developed, but also a new product for detecting the bladder cancer is developed, and the UP II antigen and the UP II antibody have positive pharmaceutical value and wide social significance.

Description

technical field [0001] The invention relates to the application of the combination of UPII antigen and UPII antibody in the preparation of products for detecting bladder cancer, and belongs to the technical field of urogenital tumor detection products. Background technique [0002] Bladder cancer is one of the tumors of the genitourinary system. In my country, bladder cancer is the tumor of the genitourinary system with the highest morbidity and mortality. The male incidence is about 7.3 / 100,000, and the female incidence is about 2.0 / 100,000. The incidence is also increasing year by year, seriously threatening people's health. Therefore, early diagnosis and long-term follow-up of patients with bladder cancer are particularly important in clinical work. [0003] The main symptom of bladder cancer is intermittent painless hematuria, which can be manifested as gross hematuria or microscopic hematuria. However, only 5%-10% of all hematuria symptoms are bladder cancer, and the ...

Claims

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Application Information

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IPC IPC(8): G01N33/574G01N33/543G01N33/58G01N21/76
CPCG01N33/57407G01N33/54326G01N33/581G01N21/76
Inventor 李少金张肄鹏张勇刚陈荣贵吴晓丽崔易航
Owner 深圳市龙华区中心医院
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