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Culture medium and culture method for thyroid cancer organoid

A technology of thyroid cancer and culture medium, which is applied in the field of biomedicine, can solve the problems of high cost of culture medium, many factors added to the culture medium, difficult thyroid cancer organoid models, etc., achieve good culture, reduce cost and operation difficulty, and improve culture Effect of success rate and number of passages

Pending Publication Date: 2021-08-20
THE SECOND PEOPLES HOSPITAL OF SHENZHEN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This kind of medium adds many factors, the cost of the medium is high, and it is difficult to obtain a long-term stable subcultured thyroid cancer organoid model in vitro

Method used

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  • Culture medium and culture method for thyroid cancer organoid
  • Culture medium and culture method for thyroid cancer organoid
  • Culture medium and culture method for thyroid cancer organoid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] Embodiment 1: the culture medium of different components

[0069] The medium was prepared according to the formula shown in Table 1.

[0070]Table 1: Media formulations with different components

[0071]

Embodiment 2

[0072] Embodiment 2: the culture medium of different components

[0073] The culture medium was prepared according to the formula shown in Table 2.

[0074] Table 2: Medium formulations with different components

[0075]

[0076]

Embodiment 3

[0077] Example 3: Culture of Thyroid Cancer Organoids

[0078] Operation: Take the culture medium with different formulations described in Example 1 and Example 2, respectively, and take the thyroid cancer tissues obtained from patients clinically, and culture thyroid cancer organoids according to the following steps:

[0079] (1) Take the thyroid cancer tissue, wash it, and cut it into pieces of about 1mm 3 Digested with type II collagenase at 37°C for 1 hour;

[0080] (2) Add Hanks solution to stop the digestion; centrifuge, discard the supernatant to obtain the first precipitate, resuspend the first precipitate with Hanks solution, filter with a 70 μm cell strainer, count the cells, and discard the supernatant Supernatant liquid, get the second precipitate, use Advanced DMEM / F12 medium to mix with the second precipitate, add matrigel to resuspend, the volume ratio of described matrigel and Advanced DMEM / F12 medium is 3:1, Inoculate, incubate for 5 minutes, and then invert...

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Abstract

The invention provides a culture medium and a culture method for a thyroid cancer organoid. The culture medium contains a basic culture medium, B27, a p38MAPK inhibitor and thyroid stimulating hormone; and the culture medium does not contain at least one of Wnt3a, R-spondin-1, noggin, a fibroblast growth factor and A83-01. When the culture medium is used for organoid culture, the cost is low, the operation difficulty is low, the culture success rate is high, and the passage number is large.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a culture medium and a culture method for culturing thyroid cancer organoids. Background technique [0002] Thyroid cancer is a malignant tumor originating from thyroid follicular epithelium or parafollicular epithelial cells. It is a common malignant tumor in the head and neck and poses a serious threat to the health and quality of life of patients. According to tumor origin and differentiation, thyroid cancer can be divided into papillary thyroid carcinoma (PTC), follicular thyroid carcinoma (FTC), medullary thyroid carcinoma (MTC) and anaplastic thyroid carcinoma (ATC). PTC and FTC are collectively referred to as differentiated thyroid cancer, and PTC accounts for about 85% to 90% of all thyroid cancers. Although most differentiated thyroid cancers can be remitted after surgery, postoperative radioactive iodine-131, and thyroid hormone suppression therapy, some patients a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/09
CPCC12N5/0693C12N2501/375C12N2501/10C12N2501/999
Inventor 黄卫人陈栋
Owner THE SECOND PEOPLES HOSPITAL OF SHENZHEN
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