Pipetting system based on machine vision technology and method thereof

Abstract

The invention discloses a pipetting system based on a machine vision technology and a method thereof, and belongs to the technical field of pipetting operation. The method comprises the following steps: acquiring an image of a second container to obtain an inner diameter value and a height value of the second container; acquiring an image of a first container, and acquiring a liquid level value inside the first container and a height value of the first container; clamping a pipette body, configuring a liquid pumping needle required by an experiment, setting the length of the liquid pumping needle required by extending into a first container to absorb liquid according to a liquid level value and a height value of the first container, and absorbing a solution to be absorbed; and allowing apipette to pump liquid at the second container, setting an included angle required by an experiment between the liquid pumping needle and the inner wall of the second container according to the inner diameter value and the height value of the second container during liquid pumping, and ensuring the liquid to flow along the inner wall of the second container. According to the invention, the liquid pumping needle operated by a mechanical arm meets the operation requirements under any condition, the intelligent degree is high, and the accuracy of liquid relief and the reliability of the operation process in the experiment process are guaranteed.

Description

technical field [0001] The invention belongs to the technical field of pipetting operation, in particular to a pipetting system and method based on machine vision technology. Background technique [0002] Each step in the human use of the pipette has specific requirements: according to the pipetting volume, select a pipette with an appropriate range; adjust the scale to an appropriate volume. When adjusting from a large volume to a small volume, the accuracy is the best; when adjusting from a small volume to a large volume, first increase it, and then return; when assembling the tip, hold the pipette and align it with the matching tip to rotate the pipette left and right And move downwards to insert the pipette tip to confirm that the pipette is tightly connected to the pipette head; when drawing liquid, press the button of the pipette to the first gear, the pipette head must be immersed in 3mm below the liquid surface, slowly release the control button, Aspirate liquid ver...

AI Technical Summary

Problems solved by technology

Although automatic pipetting can ensure the stability of the pipetting process, it is difficult to do it like a human. First, obtain the container containing the liquid to be sucked, the height of the liquid level inside the container where the liquid is to be sucked, and the place where the solution to be diluted is located. The model of the container, and then select the depth into the liquid to be sucked when aspirating according to these external factors, and the distance and inclination angle between the aspiration needle and the inner wall of the container when discharging the liquid

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