A chrysanthemum chloroplast genome ssr marker library, its acquisition method and application

Abstract

The invention discloses a chrysanthemum chloroplast genome SSR marker library, its obtaining method and application thereof. The SSR marker library includes the SSR marker sequence SEQ ID NO.1, and also includes at least one of SEQ ID NO.2‑40; the SSR marker library The method for obtaining comprises: performing SSR analysis on the chloroplast genome DNA of a chrysanthemum variety to obtain an SSR marker, splicing the SSR markers of the same variety into an SSR marker sequence, and obtaining an SSR marker library; the application of the SSR marker library is characterized in that the SSR marker The library is used to construct a phylogenetic tree or to identify chrysanthemum varieties; the SSR marker library can be used to effectively and accurately identify chrysanthemum varieties, conduct chrysanthemum genetics research and breed parent selection.

Description

technical field [0001] The invention relates to a chrysanthemum chloroplast genome SSR marker library, its obtaining method and application, and belongs to the technical field of molecular biology. Background technique [0002] Chrysanthemum plays a leading role in the exploration of flower resources, and plays a huge role in ornamental, edible and medicinal production. For chrysanthemum varieties and classification, there are related studies in the fields of traditional morphology, quantitative taxonomy, cytology, cluster analysis, etc., which provide a reference for us to understand the differences in chrysanthemum taxonomy and the genetic relationship of varieties, but these studies only focus on a small number of chrysanthemum species. Experimental analysis of chrysanthemum varieties cannot help us to identify chrysanthemums correctly and effectively. Although the identification of chrysanthemums by phenotype has the advantages of convenience and directness, the genetic...

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Problems solved by technology

For chrysanthemum varieties and classification, there are related studies in the fields of traditional morphology, quantitative taxonomy, cytology, cluster analysis, etc., which provide a reference for us to understand the differences in chrysanthemum taxonomy and the genetic relationship of varieties, but these studies only focus on a small number of chrysanthemum species. Experimental analysis of chrysanthemum varieties cannot help us to identify chrysanthemums correctly and effectively.

Although the identification of chrysanthemums by phenotype has the advantages of convenience and directness, the genetic situation becomes very complicated due to the variety of chrysanthemum varieties, small differences between varieties, parallel evolution of morphological characters, developmental variation subject to natural selection, and long-term hybridization. , leading to low identification efficiency and hindering plant reproduction and international exchanges. Therefore, there has been controversy in the classification of chrysanthemums, and it is impossible to quickly and effectively identify chrysanthemum varieties solely by morphological characteristics, cytology and other methods

[0003] At present, some scholars have developed and used molecular marker technology to study chrysanthemum at different levels, but the calculation process of these studies is complicated, suitable for small number of samples, insufficient primers or few research sites, and cannot effectively, correctly and quickly identify and distinguish most chrysanthemum species

Therefore, it is still very difficult to perfect a comprehensive and unified classification and identification method, and there are deficiencies and limitations in the classification of varieties and the study of genetic relationships.

First, the cultivation and breeding of chrysanthemums mostly start from the phenotype. Due to the similarity of the phenotypes of various varieties, it affects the acceptance of genetic traits, resulting in low efficiency of breeding new varieties

Second, there is assimilation of chrysanthemum phenotypes, small differences between varieties, and low identification accuracy, which seriously confuses chrysanthemum varieties and hinders communication and cultivation between varieties

The third is that the self-incompatibility and inbreeding of cultivated chrysanthemums lead to heterozygosity, which in turn delays genetic improvement

Fourth, the chrysanthemum classification and identification system is not perfect, which affects the tracing of its genetic relationship and origin, and the chrysanthemum is easy to hybridize to produce new variations, making its development system more complicated

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