A kind of composite material for repairing articular cartilage damage and preparation method thereof

Abstract

The invention relates to a composite material for repairing articular cartilage damage, comprising liquid collagen prepared from animal skin tissue, PRP prepared from autologous blood with a certain number of platelets, and a mass percentage concentration of 5% to 10% The calcium activator, collagen, PRP, and calcium activator are mixed in a volume ratio of (10-15): (10-15): 1, and the volume of collagen and PRP is the same, and the compound is disclosed. The preparation method of the material is to sequentially prepare collagen and PRP and mix them, and then add calcium ion activator. The preparation method of the invention is simple and easy to operate, does not require complex equipment, has a wide range of raw material sources, selects animal skin tissue and patient autologous blood, can significantly reduce costs, does not need to add exogenous thrombin, reduces the probability of adverse reactions, and can also Slows the release of growth factors in PRP.

Description

technical field [0001] The invention relates to the field of medical materials, in particular to a composite material for repairing articular cartilage damage and a preparation method thereof. Background technique [0002] Articular cartilage is a milky white, smooth and transparent tissue composed of chondrocytes and cartilage matrix. It covers the surface of the joint and can ensure the normal movement of the body's joints. Articular cartilage injury is a relatively common joint surgical disease, which can be caused by trauma, osteoarthritis, rheumatoid arthritis, and osteochondritis dissecans. Without effective treatment, it is easy to develop into knee osteoarthritis (KOA). It can lead to pain, joint deformity and even disability, seriously affecting the patient's motor function and quality of life, and at the same time increasing the patient's psychological pressure or mental burden. [0003] With the continuous development of regenerative medicine and tissue engineeri...

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Problems solved by technology

[0004] With the clinical popularization of PRP treatment of KOA, there are still some shortcomings: first, the density of PRP network structure is determined by the amount of its raw material fibrinogen, and its type depends on the total amount of thrombin and the polymerization speed

In the preparation process of traditional PRP, unpolymerized fibrin is directly discarded because it dissolves in PPP. Therefore, when thrombin is added to promote blood coagulation, the content of fibrinogen has been greatly reduced, making the fibrin network structure after polymerization The density is much lower than that of physiological blood clots. Due to the action of exogenous thrombin, the high thrombin concentration makes the polymerization rate of fibrinogen much higher than the physiological reaction, and the formed fibrin network is formed by the polymerization of four molecular fibrinogen, which is stiff The lack of elasticity is not conducive to recruiting growth factors and promoting cell migration

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